Optimization of RP-HPLC method with UV detection for determination of ursodeoxycholic acid in pharmaceutical formulations

Poposka Svirkova, Zhaklina and Arsova-Sarafinovska, Zorica and Grozdanova, Aleksandra (2020) Optimization of RP-HPLC method with UV detection for determination of ursodeoxycholic acid in pharmaceutical formulations. Macedonian Pharmaceutical Bulletin, 66 (1). ISSN 1409-8695

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Abstract

Due to the low absorptivity of bile acids, the aim of this study was to develop and validate a simple and sensitive HPLC/UV method for quantification of ursodeoxycholic acid (UDCA) in pharmaceutical formulations. Effective separation was achieved on C18 end–capped column, with gradient elution of a mobile phase composed of 0.001 M phosphate buffer (pH 2.8 ± 0.5) – acetonitrile mix, at flow rate 1.5 mL min-1, UV detection at 200 nm and injection volumes were 50 μL. The proposed HPLC method was fully validated according to the ICH guidelines and it was found to be simple, accurate, precise, and robust.

Item Type:	Article
Subjects:	Natural sciences > Chemical sciences Medical and Health Sciences > Health sciences Medical and Health Sciences > Other medical sciences
Divisions:	Faculty of Medical Science
Depositing User:	Zorica Arsova Sarafinovska
Date Deposited:	09 Mar 2021 11:54
Last Modified:	09 Mar 2021 11:54
URI:	https://eprints.ugd.edu.mk/id/eprint/27906

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