Critical points in Comet assay silver staining procedure

Milev, Mishko and Maksimova, Viktorija and Janeva, Milkica and Ruskovska, Tatjana (2020) Critical points in Comet assay silver staining procedure. Macedonian Pharmaceutical Bulletin, 66 (S1). pp. 29-30. ISSN 1857 - 8969

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Comet assay is one of the most common
methods for measuring DNA damage in eukaryotic
cells. In 1984, Ostling and Johanson demonstrated
movement of DNA strands when nuclei were
exposed to an electric field. Later, Singh et al. (1998)
modified and optimized the method using alkaline
conditions during electrophoresis, thus increasing
specificity and reproducibility of the method. In the
recent years, this assay has gained in popularity
because of its simple, economical, versatile and
sensitive procedure. This assay requires only a small
number of cells per sample and provides collection
of data at individual cell level, allowing robust
statistical analysis. According to Azqueta and
Collins (2013), there is a number of comet assay
variations, applicable to different types of samples,
such as: peripheral blood cells, cell lines, buccal
mucosa, yeasts, cancer cells and plant cells.

Item Type: Article
Subjects: Medical and Health Sciences > Basic medicine
Divisions: Faculty of Mechanical Engineering
Depositing User: Tatjana Ruskovska
Date Deposited: 18 Dec 2020 15:48
Last Modified: 18 Dec 2020 15:48

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