Koloska, Valentina and Chaloska- Ivanova, Viktorija and Panov, Sašo and Janikevik Ivanovska, Danijela and Stojkovski, Velimir and Jankulovski, Nikola (2018) Identification of pre-core and basal core promoter mutants in patients with chronic hepatitis b in the Republic of Macedonia. In: The 33rd World Congress of Biomedical Laboratory Science (IFBLS), 22-26 Sept 2018, Firenze, Italy.
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Abstract
Background: Recent development of molecular techniques has improved our understanding of the role of various
mutations of the HBV genome. Most common are mutations in the precore (PC) and basal core promoter (BCP) region,
responsible for more serious course of chronic hepatitis.
Aim of the study: was to evaluate the prevalence of PC and BCP mutants in patients with chronic hepatitis B in the
Republic of Macedonia.
METODI
Material and methods: Serum samples from 69 patients with chronic hepatitis B (47 males and 22 females, average age
49±20y.) were collected in the period from 2002-2012. All serum samples were tested for HBV, HCV and HDV infection
and immediately frozen at -70#C. According to the HBeAg status, these patients were divided in two groups: HBeAg
positive (15/69 pts or 21, 74%), and HBeAg-negative (54/69 pts or 78,26%).
Molecular examination including extraction and amplification of HBV DNA was performed. To establish if HBeAgnegative
status is related to sero-conversion, or as a consequence of viral mutations, we have used INNO-Lipa
hybridization assay from Innogenetics to identify the presence of mutations in precore and BCP region of HBV DNA.
Molecular analysis was done in 38/54 HBeAg-negative patients (28 males and 10 females).
RISULTATI
Results: The prevalence of PC mutants in 84,21% (p=0,0000) and BCP mutants in 68,42% (P=0,0033) were extremely
high in 38 examined HBeAg-negative patients. Combination of PC and BCP mutants was detected in HBV DNA of 25/38
HBeAg-negative patients (65,78%).
CONCLUSIONI
As a conclusion, HBeAg-negative stage was predominant in our patients with chronic hepatitis B and was related to
mutations in PC and BCP region.
| Item Type: | Conference or Workshop Item (Poster) |
|---|---|
| Subjects: | Medical and Health Sciences > Basic medicine Medical and Health Sciences > Clinical medicine Medical and Health Sciences > Health biotechnology Medical and Health Sciences > Health sciences Medical and Health Sciences > Other medical sciences |
| Divisions: | Faculty of Medical Science |
| Depositing User: | Danijela Janikevik |
| Date Deposited: | 03 Dec 2020 09:10 |
| Last Modified: | 03 Dec 2020 09:10 |
| URI: | https://eprints.ugd.edu.mk/id/eprint/26817 |
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