Zhang, Ziping and Wang, Yilei and Wang, Shuhong and Liu, Jingtao and Warren, Wesley and Mitreva, Makedonka and Walter, Ronald B. (2011) Transcriptome Analysis of Female and Male Xiphophorus maculatus Jp 163 A. PLoS One , 6 (4). e18379.
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Abstract
Background: Xiphophorus models are important for melanoma, sex determination and differentiation, ovoviviparity and
evolution. To gain a global view of the molecular mechanism(s) whereby gene expression may influence sexual dimorphism in Xiphophorus and to develop a database for future studies, we performed a large-scale transcriptome study.
Methodology/Principal Findings: The 454-FLX massively parallel DNA sequencing platform was employed to obtain
742,771 and 721,543 reads from 2 normalized cDNA libraries generated from whole adult female and male X. maculatus Jp
163 A, respectively. The reads assembled into 45,538 contigs (here, a "contig" is a set of contiguous sequences), of which, 11,918 shared homology to existing protein sequences. These numbers estimate that the contigs may cover 53% of the total number of Xiphophorus transcriptome. Putative translations were obtained for 11,918 cDNA contigs, of which, 3,049 amino acid sequences contain Pfam domains and 11,064 contigs encode secretory proteins. A total of 3,898 contigs were associated with 2,781 InterPro (IPR) entries and 5,411 contigs with 132 KEGG (Kyoto Encyclopedia of Genes and Genomes)
pathways. There were 10,446 contigs annotated with 69,778 gene ontology (GO) terms and the three corresponding
organizing principles. Fifty-four potential sex differentially expressed genes have been identified from these contigs. Eight and nine of these contigs were confirmed by real-time PCR as female and male predominantly expressed genes respectively. Based on annotation results, 34 contigs were predicted to be differentially expressed in male and female and 17 of them were also confirmed by real-time PCR.
Conclusions/Significance: This is the first report of an annotated overview of the transcriptome of X. maculatus and
identification of sex differentially expressed genes. These data will be of interest to researchers using the Xiphophorus model. This work also provides an archive for future studies in molecular mechanisms of sexual dimorphism and evolution, and can be used in comparative studies of other fish.
| Item Type: | Article |
|---|---|
| Subjects: | Medical and Health Sciences > Basic medicine |
| Divisions: | Faculty of Medical Science |
| Depositing User: | Mirjana Kocaleva Vitanova |
| Date Deposited: | 30 Nov 2012 14:14 |
| Last Modified: | 30 Nov 2012 14:14 |
| URI: | https://eprints.ugd.edu.mk/id/eprint/2630 |
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