Mitreva, Makedonka and Gheysen, Godelieve (2008) Exploring the transcriptome of the burrowing nematode Radopholus similis. Molecular Genetics and Genomics, 280 (1). pp. 1-17.
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Abstract
Radopholus similis is an important nematode pest on fruit crops in the tropics. Unraveling the transcriptome of this migratory plant-parasitic nematode can provide insight in the parasitism process and lead to more
eYcient control measures. For the Wrst high throughput molecular characterization of this devastating nematode, 5,853 expressed sequence tags from a mixed stage population were generated. Adding 1,154 tags from the EST division of GenBank for subsequent analysis, resulted in a
total of 7,007 ESTs, which represent approximately 3,200 genes. The mean G+C content of the nucleotides at the third codon position (GC3%) was calculated to be as high as 64.8%, the highest for nematodes reported to date.
BLAST-searches resulted in about 70% of the clustered ESTs having homology to (DNA and protein) sequences from the GenBank database, whereas one-third of them did not match to any known sequence. Roughly 40% of these latter sequences are predicted to be coding, representing putative novel protein coding genes. Functional annotation of the sequences by GO annotation revealed the abundance of genes involved in reproduction and
development, which reXects the nematode population biology. Genes with a role in the parasitism process are identiWed, as well as genes essential for nematode survival, providing information useful for parasite control.
No evidence was found for the presence of trans-spliced leader sequences commonly occurring in nematodes, despite the use of various approaches. In conclusion, we found three diVerent sources for the EST sequences: the
majority has a nuclear origin, approximately 1% of the EST sequences are derived from the mitochondrial transcriptome, and interestingly, 1% of the tags are with high probability derived from Wolbachia, providing the first molecular indication for the presence of this endosymbiont in a plant - parasitic nematode.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Expressed sequence tag analysis Trans-spliced leader Parasitism G + content Wolbachia Endoparasitic migratory nematode |
| Subjects: | Medical and Health Sciences > Other medical sciences |
| Divisions: | Faculty of Medical Science |
| Depositing User: | Marija Kalejska |
| Date Deposited: | 30 Nov 2012 14:18 |
| Last Modified: | 30 Nov 2012 14:18 |
| URI: | https://eprints.ugd.edu.mk/id/eprint/2616 |
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