An expressed sequence tag analysis of the life-cycle of the parasitic nematode Strongyloides ratti

Thompson, Fiona J. and Mitreva, Makedonka and Barker, Gary L.A. and Martin, John and Waterston, Robert H. and McCarter, James P. and Viney, Mark E. (2005) An expressed sequence tag analysis of the life-cycle of the parasitic nematode Strongyloides ratti. In: Molecular and Biochemical Parasitology. Elsever, pp. 32-46.

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14,761 expressedsequencetags (ESTs) were generated, representing five stages during the parasitic and free-living phases of the life-cycle of the parasiticnematodeStrongyloidesratti. These ESTs formed 4152 clusters, of which 97% contained 10 or fewer ESTs and 66% were singletons. These 4152 clusters are likely to represent approximately 20% of S. ratti's genes. The clusters’ consensus sequences were used to assign each cluster to one of three databases: (i) Caenorhabditis elegans and C. briggsaesequences; (ii) other nematodesequences; (iii) non-nematodesequences. This approach has identified putative nematode-specific genes, that may be targets for developing approaches for parasiticnematode control. Approximately 25% of the clusters have no significant alignments and may therefore represent novel genes. The EST representation between the libraries was used to analyse stage-specific or -biased expression in silico. This showed that 81% of clusters are present in only one library and 12% are present in any two libraries, indicating substantial stage-specificity of gene expression. The 30-most abundantly expressed clusters were analysed in further detail. Many of these have significantly different parasitic- or free-living-specific or -biased expression. Many of the parasitic-specific genes are, as yet, uncharacterised: one of these represents 25% of all ESTs obtained from the parasitic stage.

Item Type: Book Section
Uncontrolled Keywords: ESTs; Gene expression; Genomics; Nematode; Parasite; C. elegans
Subjects: Medical and Health Sciences > Other medical sciences
Divisions: Faculty of Medical Science
Depositing User: Marija Kalejska
Date Deposited: 30 Nov 2012 10:49
Last Modified: 30 Nov 2012 10:49

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