A Catalog of Reference Genomes from the Human Microbiome

Nelson, Karen and Weinstock, George M and Highlander, SK and Worley, KC and Creasy, HH and Wortman, JR and Rusch, DB and Mitreva, Makedonka and Grupa avtori (2010) A Catalog of Reference Genomes from the Human Microbiome. Science, 328 (5981). pp. 994-999.

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Abstract

The human microbiome refers to the community of microorganisms including prokaryotes, viruses
and microbial eukaryotes that populate the human body. The National Institutes of Health launched
an initiative that focuses describing the diversity of microbial species associated with health and
disease. The first phase of this initiative includes the sequencing of hundreds of microbial reference
genomes, coupled to metagenomic sequencing from multiple body sites. Here we present results
from an initial reference genome sequencing of 178 microbial genomes. From 547,968 predicted
polypeptides that correspond to the gene complement of these strains “novel” polypeptides that had
both unmasked sequence length > 100 amino acids and no BLASTP match to any non-reference
entry in the nr subset were defined. This analysis resulted in a set of 30,867 polypeptides, of which
29,987 (~97%) were unique. In addition, this set of microbial genomes allows for ~ 40% of random
sequences from the microbiome of the gastrointestinal tract to be associated with organisms based
on the match criteria used. Insights into pan-genome analysis suggest that we are still far from
saturating microbial species genetic datasets. In addition, the associated metrics and standards used
by the group for quality assurance are presented.

Item Type: Article
Subjects: Medical and Health Sciences > Basic medicine
Divisions: Faculty of Medical Science
Depositing User: Mirjana Kocaleva
Date Deposited: 30 Nov 2012 10:37
Last Modified: 07 Oct 2013 09:56
URI: https://eprints.ugd.edu.mk/id/eprint/2579

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