The canine hookworm genome: analysis and classification of Ancylostoma caninum survey sequences

Abubucker, Sahar and Martin, John and Yin, Yong and Fulton, Lucinda and Yang, Shiaw-Pyng and Hallsworth-Pepin, Kym and Johnston, J. Spencer and Hawdon, John and McCarter, James P. and Wilson, Richard K. and Mitreva, Makedonka (2008) The canine hookworm genome: analysis and classification of Ancylostoma caninum survey sequences. Mol Biochem Parasitol, 157 (2). pp. 187-192.

[thumbnail of nihms39185.pdf]

Download (386kB) | Preview


Hookworms infect nearly a billion people. The Ancylostoma caninum hookworm of canids is a model
for studying human infections and information from its genome coupled with functional genomics
and proteomics can accelerate progress towards hookworm control. As a step towards a full-scale
A. caninum genome project, we generated 104,000 genome survey sequences (GSSs) and determined
the genome size of the canine hookworm. GSSs assembled into 57.6 Mb of unique sequence from a
genome that we estimate by flow cytometry of isolated nuclei to be 347±1.2 Mb, substantially larger
than other Rhabditina species. Gene finding identified 5,538 genes in the GSS assembly, for a total
of 9,113 non-redundant A. caninum genes when EST sequences are also considered. Functional
classifications of many of the 70% of genes with homology to genes in other species are provided
based on Gene Ontology and KEGG associations and secreted and membrane-bound proteins are
also identified.

Item Type: Article
Uncontrolled Keywords: hookworm; Ancylostoma caninum; genome survey sequences; expressed sequence tags; genome; comparative genomics
Subjects: Medical and Health Sciences > Basic medicine
Divisions: Faculty of Medical Science
Depositing User: Mirjana Kocaleva
Date Deposited: 30 Nov 2012 10:35
Last Modified: 20 Dec 2012 09:38

Actions (login required)

View Item View Item