Detection of putative secreted proteins in the plant-parasitic nematode Heterodera schachtii

Mitreva, Makedonka and Bird, David M. and Cheysen, Godelieve (2005) Detection of putative secreted proteins in the plant-parasitic nematode Heterodera schachtii. Parasitol Res , 98. pp. 414-424.

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The beet cyst nematode Heterodera schachtii is an important pathogen worldwide, but its molecular characterization has been limited to studying individual genes of interest. We undertook a high-throughput genomic
approach and drastically increased the number of available sequences for this parasite. A total of 2,662 expressed sequence tags were grouped into 1,212 clusters representing a nonredundant catalog of H. schachtii genes. Im-
plementing a bioinformatic workflow, we identified 50 sequences coding for candidate secreted proteins. All of these contain a putative signal peptide required for entry into the secretory pathway and lack any transmembrane
domain. Included are previously postulated cell-wall- degrading enzymes and other parasitism-related genes. Moreover, we provide the first report of an arabinogalactan endo-1,4-β-galactosidase enzyme (EC in ani-
mals. As sequence data increase at a rapid rate, developinghigh-throughput genomic screening is a necessity. The in silico approach described here is an effective way to identify putative secreted proteins and prioritize candidates
for further studies.

Item Type: Article
Subjects: Medical and Health Sciences > Other medical sciences
Divisions: Faculty of Medical Science
Depositing User: Marija Kalejska
Date Deposited: 30 Nov 2012 08:01
Last Modified: 30 Oct 2014 13:45

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