Investigating hookworm genomes by comparative analysis of two Ancylostoma species

Mitreva, Makedonka and McCarter, James P. and Arasu, Prema and Hawdon, John and Martin, John and Dante, Mike (2005) Investigating hookworm genomes by comparative analysis of two Ancylostoma species. BMC Genomics. pp. 1-19.

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Background: Hookworms, infecting over one billion people, are the mostly closely related major
human parasites to the model nematode Caenorhabditis elegans. Applying genomics techniques to
these species, we analyzed 3,840 and 3,149 genes from Ancylostoma caninum and A. ceylanicum.
Results: Transcripts originated from libraries representing infective L3 larva, stimulated L3,
arrested L3, and adults. Most genes are represented in single stages including abundant transcripts
like hsp-20 in infective L3 and vit-3 in adults. Over 80% of the genes have homologs in C. elegans,
and nearly 30% of these were with observable RNA interference phenotypes. Homologies were
identified to nematode-specific and clade V specific gene families. To study the evolution of
hookworm genes, 574 A. caninum / A. ceylanicum orthologs were identified, all of which were found
to be under purifying selection with distribution ratios of nonsynonymous to synonymous amino
acid substitutions similar to that reported for C. elegans / C. briggsae orthologs. The phylogenetic
distance between A. caninum and A. ceylanicum is almost identical to that for C. elegans / C. briggsae.
Conclusion: The genes discovered should substantially accelerate research toward better
understanding of the parasites' basic biology as well as new therapies including vaccines and novel

Item Type: Article
Subjects: Medical and Health Sciences > Basic medicine
Divisions: Faculty of Medical Science
Depositing User: Mirjana Kocaleva
Date Deposited: 29 Nov 2012 14:21
Last Modified: 04 Oct 2013 11:16

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