Gene discovery in the adenophorean nematode Trichinella spiralis: an analysis of transcription from three life cycle stages

Mitreva, Makedonka and Jasmer, Douglas P. and Appleton, Judith and Martin, John and Dante, Mike and Wylie, Todd and Clifton, Sandra W. and Waterston, Robert H. and McCarter, James P. (2004) Gene discovery in the adenophorean nematode Trichinella spiralis: an analysis of transcription from three life cycle stages. Molecular and Biochemical Parasitology, 137 (2). pp. 277-291.

Full text not available from this repository.


Expressed sequence tags (ESTs) were produced from cDNA libraries for immature L1, mature muscle larva and adult stages of the adenophorean nematode Trichinella spiralis. 10,130 ESTs were grouped into 3454 gene clusters. The clusters represent a conservative estimate of 3262 unique genes. Interspecific comparisons of the predicted proteins support an ancient divergence of clade I nematodes from other nematodes in the phylum Nematoda. Furthermore, apparent clade I or Trichocephalida-specific proteins were identified, which may include molecular determinants important in the evolution of these species. Similarity matches identified 463 C. elegans genes homologs that confer phenotypes by RNA interference. Classification of predicted proteins suggested diverse cellular, metabolic and extracellular functions, significantly expanding the dataset of T. spiralis proteins with prospective, and potentially critical, functions. Several lines of evidence suggested stage-specific expression of certain genes beyond those previously identified. Evidence was obtained for the existence of large gene families encoding isoforms of known secreted proteins, such as p43 and TspE1. Unexpectedly, diverse isoforms of the muscle larva p43 gene appear to be expressed by immature L1. Proteinases, kinases, antioxidant proteins and enzymes involved in glycan synthesis are implicated in T. spiralis interactions with its hosts. Numerous genes were identified that encode predicted proteins in these categories. The genes discovered, when put into context of functional classification, stage of expression, and biology of the parasite, should substantially enhance experimental potential for research on this parasite.

Item Type: Article
Uncontrolled Keywords: Trichinella; Comparative genomics;Expressed sequence tags; Gene expression;Parasite;Nematode
Subjects: Medical and Health Sciences > Basic medicine
Divisions: Faculty of Mechanical Engineering
Depositing User: Mirjana Kocaleva
Date Deposited: 29 Nov 2012 14:20
Last Modified: 29 Nov 2012 14:20

Actions (login required)

View Item View Item