Bermanec, Vladimir and Paradžik, Tina and P.Kazazić, Snježana and Venter, Chantelle and Hrenovic, Jasna and Vujaklija, Dusica and Duran, Robert and Boev, Ivan and Boev, Blazo (2020) Novel arsenic hyper-resistant bacteria from an extreme environment, Crven Dol mine, Allchar, North Macedonia. Journal of Hazardous Materials. ISSN 0304-3894
Full text not available from this repository.Abstract
Novel hyper-resistant bacteria were isolated from the Crven Dol mine (Allchar, North Macedonia), arsenic-rich extreme environment. Bacteria were recovered from a secondary mineral mixture, an alteration of hydrothermal realgar rich in arsenates (pharmacolite, hornesite, and talmessite). The sample was recovered from the dark part of the mine at 28 m depth. Three bacterial strains and a bacterial consortium were isolated for their capacity to survive exposure to 32 g/L (209 mM) of arsenite, and 176 g/L (564 mM) of arsenate. The 16S rRNA gene analysis identified bacterial isolates as Stenotrophomonas sp. and two Microbacterium spp. This analysis also revealed that bacterial consortium comprise two Bacteriodetes exhibiting similarity to Olivibacter ginsengisoli and to uncultured bacterium, and one γ-proteobacteria with similarity to Luteimonas sp. Among all isolates Stenotrophomonas sp. exhibited the highest tolerance to As compound as well as the capacity to accumulate As inside the cells. Analysis of genes involved in As-resistance showed that recovered isolates possess the genes encoding the ArsB, Acr3(1) and Acr3(2) proteins, indicating that at least a part of their resistance could be ascribed to As-efflux systems described in isolates obtained from human-polluted environments.
| Item Type: | Article |
|---|---|
| Impact Factor Value: | 9.038 |
| Uncontrolled Keywords: | Biogeochemistry, arsenate, arsenite, hyper As-resistance, As-transporters, 16S rRNA |
| Subjects: | Natural sciences > Earth and related environmental sciences Natural sciences > Other natural sciences |
| Divisions: | Faculty of Natural and Technical Sciences |
| Depositing User: | Biljana Kosturanova |
| Date Deposited: | 14 Jul 2020 07:41 |
| Last Modified: | 14 Jul 2020 07:41 |
| URI: | https://eprints.ugd.edu.mk/id/eprint/24223 |
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