Method of single cell electrophoresis – research opportunities

Ruskovska, Tatjana (2019) Method of single cell electrophoresis – research opportunities. In: Second congress of students of general medicine, 09-10 May 2019, Stip, Republic of North Macedonia.

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Genotoxins are agents of various nature that can cause DNA damage. An important characteristic of the living cells is the ability to repair the damage, but it is not unlimited. If DNA damage is not repaired, cancer, cell death or transmission of the damage to the next generations can occur. For this reason, it is particularly important to detect the presence of agents with genotoxic properties in the environment, and then respond appropriately for protection against their harmful effects.
The main goal of the scientific research project entitled: “A Field Detector for Genotoxicity from CBRN and Explosive Devices”, funded by NATO, is development of such a detector, where living cells will be used as a biosensor for the presence of genotoxins of various nature. The Faculty of Medical Sciences participates in this international project, in cooperation with the partners from Bulgaria, USA and Turkey.
One of the most popular methods for detecting the DNA damage is the method of single cell electrophoresis in agarose gel, or commonly known as comet assay. Within the NATO project activities, this method is implemented in the Biochemistry Laboratory at the Faculty of Medical Sciences. The method is relatively simple, quite sensitive, and has diverse applications. It can be used within basic research on the mechanisms of DNA damage and repair, for testing of genotoxicity of new drugs, cosmetic products and nanomaterials, for research in the field of ecotoxicology, for human biomonitoring, as well as within human intervention studies with plant bioactives and nutraceuticals.
Within this lecture, the basic principles of the comet assay will be presented, as an introduction to the training course entitled: “Comet assay – theoretical and practical aspects”, supported by the NATO project “A Field Detector for Genotoxicity from CBRN and Explosive Devices”.

Item Type: Conference or Workshop Item (Lecture)
Subjects: Medical and Health Sciences > Basic medicine
Medical and Health Sciences > Clinical medicine
Divisions: Faculty of Medical Science
Depositing User: Tatjana Ruskovska
Date Deposited: 16 Jan 2020 11:30
Last Modified: 16 Jan 2020 11:30

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