The importance of nuclear division index in biomonitoring human studies

Velickova, Nevenka and Milev, Mishko (2019) The importance of nuclear division index in biomonitoring human studies. In: 1st Congress of Geneticists in Bosnia and Herzegovina with international participation, 2-5 Oct 2019, Sarajevo, BiH.

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Introduction: Radiation as a physical agents can cause major alteration to the genetic material, chromosomal instability and cell damage. In this study the micronucleus assay (MN) is being applied for biomonitoring the hospital workers exposure to ionizing radiation or in vitro genotoxicity testing of radiation as a predictor of cancer risk, because chromosomal mutation and cell alteration is the main reason for carcinogenesis. The frequency of micronuclei (MNi) and nuclear division index (NDI) are very important factors and usefull indicators for genotoxicity, mitogenic response and proliferative status of the lymphocytes.
Material and methods: It was a prospective observational study when MN assay is being applied on control and exposed group (hospital workers with different time of exposure and work activity).
The results of this study confirme the high frequency of MNi in hospital workers with long time of exposure and presence of nucleoplasmic bridges (NPBs), nuclear buds (NBUDs) as biomarkers of DNA miss repair complexes. We evaluated when NDI value is lowest than 1.0 all of peripheral lymphocytes are mononucleated, when NDI value is close to 2.0 most of the lymphocytes are binucleated, when NDI value is greater than 2.0 therefore most of the lymphocytes contain more than two nuclei. Our observation confirme, when we apply MN on peripheral lymphocytes the NDI value is better to be close to 2. Also, the NDI value is high in hospital workers with long time of exposure than in control group.
Conclusions: The frequency of MNi is high in peripheral lymphocytes in exposed hospital workers than in control group. NDI is useful parameter and could provide better observations for genotoxicity of radiation or other agents in similar studies which biomonitoring the human cells using MN assay.

Item Type: Conference or Workshop Item (Poster)
Subjects: Medical and Health Sciences > Basic medicine
Divisions: Faculty of Medical Science
Depositing User: Nevenka Velickova
Date Deposited: 25 Oct 2019 08:35
Last Modified: 25 Oct 2019 08:35

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