Getting insight into enzymes kinetics and thermodynamics via theoretical models in protein-film square-wave voltammetry

Janeva, Milkica and Maksimova, Viktorija and Gulaboski, Rubin (2019) Getting insight into enzymes kinetics and thermodynamics via theoretical models in protein-film square-wave voltammetry. In: 7th International Symposium on Sensor Science, Napoli, Italy, 9-11 May 2019.

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We present in this work several relevant theoretical models of Protein-film square-wave voltammetry of uniformly adsorbed molecules of redox enzymes. Theoretical consideration of several one-electron step and two-electron step mechanisms that are coupled to preceding, follow up or regeneration (catalytic) chemical steps under conditions of square-wave voltammetry reveal many new aspects, especially by enzymatic electrode reactions featuring fast electron transfer. We show in this work that the phenomena of “split net-SWV peak” and “quasireversible maximum”, which are typical for simple protein-film reactions studied in square-wave voltammetry, are strongly affected by kinetics and thermodynamics of preceding, follow-up, or regenerative chemical steps. While we present plenty of relevant voltammetric situations useful for recognizing all relevant and most common protein-film mechanisms in square-wave voltammetry, we also propose several new approaches to get access to kinetics and thermodynamics of chemical steps in all those mechanisms. Most of the results in this work throw new insight into the features of protein-film systems that are coupled with chemical reactions.

Item Type: Conference or Workshop Item (Poster)
Uncontrolled Keywords: Keywords: enzymatic voltammetry; square-wave voltammetry; surface ECrev mechanism; kinetics of follow-up chemical reactions; standard rate constant of electron transfer.
Subjects: Medical and Health Sciences > Other medical sciences
Divisions: Faculty of Medical Science
Depositing User: Milkica Janeva
Date Deposited: 10 Jun 2019 07:26
Last Modified: 10 Jun 2019 07:26

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