Probing the redox activity of T-lymphocytes deposited at electrode surfaces with voltammetric methods

Bogeski, Ivan and Mirceski, Valentin and Hoth, Markus (2008) Probing the redox activity of T-lymphocytes deposited at electrode surfaces with voltammetric methods. Clinical Chemical Laboratory Medicine, 46 (2). ISSN 14346621

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Abstract

Background: Reactive oxygen species and redox
signaling play an important role in the regulation of
many vital biological processes. However, they are
also tightly connected with many pathological conditions.
The detection and evaluation of these signaling
events are very often accompanied with great difficulties.
In this article, we describe the development of
a novel electrochemically-based technique for monitoring
the cellular redox state.
Methods and results: T-cells were attached on the
surface of a working electrode, which was modified
with 2-palmitoylhydroquinone as a redox mediator.
Using cyclic voltammetry, we were able to indirectly
(via the redox mediator) monitor an electron transport
from the cells towards the working electrode, which
enabled us to evaluate the redox activity of the cells.
Conclusions: This new technique is rather simple and
sensitive and may be used in the future as a valid
diagnostic procedure in various branches of biomedical
science.

Item Type:	Article
Subjects:	Medical and Health Sciences > Basic medicine Natural sciences > Chemical sciences
Divisions:	Faculty of Medical Science
Depositing User:	Ivan Bogeski
Date Deposited:	27 Nov 2012 10:43
Last Modified:	27 Nov 2012 10:43
URI:	https://eprints.ugd.edu.mk/id/eprint/2198

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