Square-wave voltammetry of 5-fluorouracil

Mirceski, Valentin and Gulaboski, Rubin and Jordanoski, Blagoja and Komorsky-Lovrić, Šebojka (2000) Square-wave voltammetry of 5-fluorouracil. Journal of Electroanalytical Chemistry, 490 (1-2). pp. 37-47. ISSN 15726657

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The redox reaction of 5-fluorouracil (FU) at a hanging mercury drop electrode (HMDE) is studied by means of square-wave
voltammetry (SWV). It is demonstrated that the redox reaction proceeds according to the scheme: L2�(aq)vL2�(ads)�
Hg(l)vHgL(s)�2e�, which involves both chemisorption of FU on the electrode surface and creation of a sparingly soluble
compound with the electrode material. The overall response exhibits properties of a surface process in which both the reactant and
the product of the redox reaction are immobilized on the electrode surface. The square-wave voltammetric response of FU
possesses features typical of surface confined processes such as ‘split SW peaks’ and a ‘quasi-reversible maximum’. The proposed
electrode mechanism is studied theoretically. The numerically calculated response under conditions of SWV is in qualitative
agreement with the experimental data. Comparing the theoretical and the experimental data, the kinetic parameters of the redox
reaction investigated are estimated. The standard rate constant appears to be within the interval 545ks:s�15108, the adsorption
constant is K�10 cm�1, and the transfer coefficient is a�0.5490.01. The effect of the Cu(II) ions on the adsorptive SWV
response of FU is discussed from an analytical point of view. It is demonstrated that SWV is a particularly appealing technique,
which enables determination of FU at an ultra-trace concentration level. A linear calibration plot was established at 10�11 mol
l�1 concentration level with a correlation coefficient of R2�0.992. The detection limit is 7.7�10�12 mol l�1. The reproducibility
of the results in terms of the relative standard deviation ranges from 0.9 to 3.2%

Item Type: Article
Uncontrolled Keywords: 5-Fluorouracil, biosensor, square-wave voltammetry, electrochemistry, anticanerogenic drugs, kinetic measurements
Subjects: Medical and Health Sciences > Basic medicine
Natural sciences > Chemical sciences
Natural sciences > Matematics
Natural sciences > Physical sciences
Divisions: Faculty of Agriculture
Depositing User: Rubin Gulaboski
Date Deposited: 31 Oct 2012 23:10
Last Modified: 05 Nov 2012 10:59
URI: https://eprints.ugd.edu.mk/id/eprint/218

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