Roganovic-Zafirova, Danica and Velickova, Nevenka (2010) Comparative microscopy analysis of liver and gonads of the Prespa roach (rutilus rubilio prespensis karaman) and the Prespa barbell (barbus plebejus prespensis karaman) in conditions of polluted water in Prespa Lake. In: 4TH International symposium of ecologists of Montenegro, 6 – 10 October, Budva, Montenegro.
![[thumbnail of ISEM 4_Montenegro.pdf]](/style/images/fileicons/text.png) |
Text
ISEM 4_Montenegro.pdf Download (1MB) |
Abstract
The aim of this study was to make microscopy analysis of the liver tissue, specially with an accent to liver’s parenchyma. Stereological analysis to the nucleus of the hepatocytes and semiquantitative analysis to the presence of iron of the hepatocytes was carried out.
Material and methods: In this study a microscopy analysis of liver was carried out at two fish species from Prespa lake: the Prespa roach (Rutilus rubilio prespensis Karaman) and the Prespa barbell (Barbus plebejus prespensis Karaman) , with an aim to define the dynamics of the liver parenchyma during the reproductive cycle and eventually the inter- species difference in this process, and to confine normal phisiological changes in the liver during the reproductive cycle from the liver lessions caused by the toxic effects of the polluted area. 91 fishes of two species were selected and grouped by their sex and the stage of the reproductive cycle according to the state of the gonads.
Results: The results indicated that the hepatocytes undergo a sequence of changes which are synchronized with the reproductive cycle, of the both of fish species. During the pre-vitellogenesis hepatocytes are higher, and they are marked by massive glicogen accumulation, since the end of endogene vitellogenesis and during exogene vitellogenesis they go through a phase of intensive protein synthesis, at the end of vitellogenesis and before spawning they get into a phase of involution and massive autophagocitosis of their cytoplasmic organelles. Impressive and clearly expressed is the polarity in hepatocytes during their differentiation. Conclusion: Considering the dynamics of liver’s parenchyma during the reproductive cycle, we observed interspecies difference between multi-spawning of Prespa barbell, and uni-spawning Prespa roach. The stereological analysis indicated significant culmination of all nucleus parameters, of vitellogenesis of females of both species, and in the beginning of spermatogeneze of the male Prespa roach. Perl's prussian blue staining revealed the presence of iron in hepatocyte cytoplasma, during the certain stages of reproductive cycle, or in association of liver lesions in both fish species, Prespa barbell and Prespa roach. In the liver of several fish, we detected intraparenchymal infiltration with macrophages and leukocytes, fibrosis around the blood vessels and biliary ducts, fungal and Capilaria’s infection assosieted with necrosis.
| Item Type: | Conference or Workshop Item (Poster) |
|---|---|
| Subjects: | Natural sciences > Biological sciences |
| Divisions: | Faculty of Natural and Technical Sciences |
| Depositing User: | Nevenka Velickova |
| Date Deposited: | 26 Nov 2012 13:42 |
| Last Modified: | 26 Nov 2012 13:42 |
| URI: | https://eprints.ugd.edu.mk/id/eprint/2131 |
Actions (login required)
 |
View Item |