Voltammetric Sensors for Hydrogen Peroxide Detection in Living Cells

Meckaroska, Elena and Ristovska, Isidora and Janeva, Milkica and Kokoskarova, Pavlinka (2018) Voltammetric Sensors for Hydrogen Peroxide Detection in Living Cells. In: Firts Congress of Students of General Medicine, Goce Delcev University, 09-10 May 2018, Stip, Macedonia.

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Hydrogen Peroxide (H2O2) is one of the most important small molecules involved in various signalling processes at all living systems. Hydrogen peroxide is commonly created as a by-product in the respiration processes. There is significant amount of scientific information showing the involvement of H2O2 in signalling of stress responses, and in many other redox-signalling related processes. In most of the mechanisms featuring redox-signalling in the cells, it is confirmed that thiol-containing molecules play important role, usually undergoing a redox transformation in reactions with H2O2. The redox chemistry of H2O2 is quite complex, since it can be involved in variety of reactions. Depending on the pH, H2O2 can be seen as a substance with highly oxidative potential, but also as a compound with reductive properties. The two most simple 2-electron scenarios of oxidation and reduction of H2O2 can be described by following reactions:
H2O2+2H++2e−⟶2H2 E°= +1.534 V vs. SCE (pH of 7.00)
2H++O2+2e−⟶ H2O2 E°= +0.440 V vs. SCE (pH of 7.00)
In this work, we show several ways of designing voltammetric sensors for hydrogen peroxide quantification. These methods can be designed for direct detection and quantification of hydrogen peroxide, but more efficient are the approaches in the so-called Electrocatalytic-Regenerative (EC’) electrode mechanism. With the methods presented here, detection of hydrogen peroxide in micro-to-milimolar range is possible.

Item Type: Conference or Workshop Item (Poster)
Subjects: Medical and Health Sciences > Basic medicine
Natural sciences > Chemical sciences
Natural sciences > Matematics
Natural sciences > Physical sciences
Divisions: Faculty of Medical Science
Depositing User: Rubin Gulaboski
Date Deposited: 12 Nov 2018 09:13
Last Modified: 12 Nov 2018 09:13
URI: https://eprints.ugd.edu.mk/id/eprint/20799

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