Evrosimovska, Biljana and Dimova, Cena (2017) Matrix metalloproteinases in oral diseases (with accent on matrix metalloproteinase-1 in chronic periapical lesions). Journal of Hygienic Engineering and Design, 20 (3). pp. 54-59. ISSN 1857- 8489
Preview |
Text
04. Full paper - Biljana Evrosimovska.pdf - Published Version Download (156kB) | Preview |
Abstract
Matrix metalloproteinases (MMPs) are zinc and calcium
dependent enzymes capable of degrading almost
all extracellular matrix and basement membrane components.
This group of proteolytic enzymes believed
to be implicated in the breakdown of extracellular matrix
in normal physiological processes, as well as, plays
an important role in many destructive pathological
oral processes, such as periodontal tissue destruction,
root caries, tumor invasion, and chronic periapical inflammation
(CPL). The aim of this study was to analyze
polymorphism in the gene MMP-1 and their association
and influence on clinical manifestation of chronic
periapical lesions in order to provide new advances
regarding the involvement of MMPs in various oral diseases
associated with the inflammatory process.
A total of 240 unrelated Macedonian subjects were included
in the present study. Polymorphism -1607 1G/2G
in the gene MMP-1 detected with restriction enzymes
AluI, XmnI and polymorphism -519 A/G in the gene
MMP-1 detected with restriction enzyme KpnI was
study in 120 patients with CPL and 120 controls without
any signs of chronic or acute inflammatory process in
the jaw. The amplification of the region of selected gene
was made with polymerase chain reaction-restriction
fragment length polymorphism (PCR-RFLP).
Our results showed that there was a differences in the
allele and genotype frequencies of the MMP-1 polymorphism
between patients with CPL and controls
(p<0.05). Also this study suggests that MMP-1 polymorphism
-1607 1G/2G detected with restriction enzymes
AluI, XmnI was a risk for expression of CPL (OR
= 18.38 < 4.06 < OR < 115.46; OR = 7.73 < 3.1 < OR <
19.55) and MMP-1 polymorphism -519 A/G detected
with restriction enzyme KpnI was a risk for expression
of CPL (OR = 12.11 < 4.64 < OR < 32.30).
Detection of this genetic polymorphism is relevant for
obtaining providential treatment of patients who are
at high risk of chronic periapical inflammation.
Key words: Matrix metalloproteinases, Oral diseases,
Polymerase chain reaction, Gene mutation.
| Item Type: | Article |
|---|---|
| Subjects: | Medical and Health Sciences > Basic medicine Medical and Health Sciences > Clinical medicine Medical and Health Sciences > Health biotechnology Medical and Health Sciences > Health sciences Medical and Health Sciences > Other medical sciences |
| Divisions: | Faculty of Medical Science |
| Depositing User: | Cena Dimova |
| Date Deposited: | 12 Dec 2017 11:13 |
| Last Modified: | 12 Dec 2017 11:13 |
| URI: | https://eprints.ugd.edu.mk/id/eprint/18602 |
Actions (login required)
 |
View Item |