Determination of phytol content in some vegetables and fruits

Kostik, Vesna and Bauer, Biljana (2017) Determination of phytol content in some vegetables and fruits. In: MESMAP-3, 13-16 Apr 2017, Merit Park Hotel, Girne - Turkish Republic of Northern Cyprus.

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Phytol is an acyclic diterpene alcohol which is formed by enzymatic degradation of chlorophyll in plants [1]... Phytol is widely used in the synthesis of vitamin and E and K, in the fragrance industry, cosmetics, as a food additive etc
The use of phytol in the human body is essential in activating enzymes that have a positive effect on the production of insulin and enzymes that decrease blood cholesterol [2].
The present study investigates phytol content in samples of fresh fruits and vegetables grown in the Republic of Macedonia. For that purpose a total of 170 samples of different fresh vegetables: tomato, cucumber, paprika, potato, beans, carrot, beetroot, leek, eggplant, zucchini and okra; fruits: apples, grapes, plums, peaches, watermelon and melon were tested. Extraction of the plants was performed with acetone followed by re-extraction with petroleum ether (40-60) and purification on cartridges packed with polymerically bonded ethylenediamine-N-propyl phase (PSA). Identification and determination of phytol was performed with gas chromatography – tandem mass spectrometry (GC-MS). The results of the study showed that content of phytol in the extract of analyzed fruit samples was in the range from less than 0.01% (w/w) for plums to 3.5% (w/w) for grapes. Vegetables with low content of phytol were: eggplant, tomato, potato, carrot, beetroot, and beans. Cucumber was the crop with highest phytol content with average value of 26.9% (w/w). The extracts obtained from leek contained 7.2% (w/w) phytol, and the extracts obtained from okra contained 3.8% (w/w) phytol.
Results of our investigations showed that vegetables with higher content of chlorophyll as cucumber and leek contain phytol in higher concentration.

Keywords: phytol, vegetables, fruits, gass chromatography tandem mass spectrometry (GC-MS)

Item Type: Conference or Workshop Item (Poster)
Subjects: Medical and Health Sciences > Other medical sciences
Divisions: Faculty of Medical Science
Depositing User: Vesna Kostik
Date Deposited: 14 Aug 2017 13:15
Last Modified: 14 Aug 2017 13:15

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