Atanasova, Biljana (2015) Fauna of cicadas (Hemiptera:Auchenorrhyncha)in the vineyards of macedonia and their role in the epidemiology of 'Candidatus phytoplasma solani'. PhD thesis, University of Belgrade, Faculty of Biology.
Preview |
Text
Teza.pdf Download (766kB) | Preview |
Abstract
Bois noir (BN), induced by stolbur phytoplasma, is an important grapevine yellows disease that causes severe damage in viticultural regions throughout the Euro-Mediterranean basin. Epidemiological survey to determine potential insect vectors and the main reservoir plants of BN phytoplasma in Macedonian vineyards was undertaken between 2012 and 2013 in southeastern part of the country. The study of the species diversity from the suborder Auchenorrhycha revealed the presence of 27 species, belonging to 6 families. The most numerous was family Cicadellidae with 21 species, followed by family Cixiidae with 2 species, Aphrophoridae, Delphacidae, Dyctiopharidae and Issidae with only one species recorded. Our study revealed the high abundance of the principal vector of stolbur phytoplasma, the planthopper Hyalesthes obsoletus, while the second documented vector of BN, Reptalus panzeri, was not recorded in Macedonian vineyards. A molecular characterization performed by sequence and/or RFLP typing of tuf, vmp1 and stamp genes, was used to gain detailed insight into the molecular diversity of the stolbur phytoplasma isolates associated with grapevine, tentative reservoir plants (Urtica dioica and Convolvulus arvensis) and H. obsoletus associated with them. Among 91 stolbur isolates detected in diverse plant and insect hosts 3 tuf, 5 vmp1 and 11 distinct stamp genotypes were identified. Overall, twelve comprehensive genotypes of stolbur phytoplasma were detected according to tuf/vmp1/stamp genotyping. The highest diversity of genotypes was detected among the isolates from H. obsoletus associated with U. dioica, of which the most frequent genotype was tuf-ab/V18/M1 (43%). Tuf-b/V2-TA/STOL comprehensive genotype was found in 33% of naturally infected grapevines. Two stolbur phytoplasma genotypes were associated with U. dioica: (i) tuf-ab/V18/M1 (60%) and tuf-a/V3/M4 (40%), while only one genotype (tuf-b/V2-TA/Rqg50) was associated with C. arvensis.
| Item Type: | Thesis (PhD) |
|---|---|
| Uncontrolled Keywords: | grapevine yellows, Bois noir, molecular epidemiology, Hyalesthes obsoletus, stamp variability, stolbur phytoplasma |
| Subjects: | Natural sciences > Biological sciences Agricultural Sciences > Other agricultural sciences |
| Divisions: | Faculty of Agriculture |
| Depositing User: | Biljana Atanasova |
| Date Deposited: | 01 Mar 2017 10:04 |
| Last Modified: | 01 Mar 2017 10:04 |
| URI: | https://eprints.ugd.edu.mk/id/eprint/17514 |
Actions (login required)
 |
View Item |