Velickova, Nevenka (2016) Cytological monitoring of human lymphocites, the main tool in environmental research. In: Protection of natural resources and environmental managment: The main tools for sustainability, 10-13 Nov 2016, Bucharest, Romania.
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Abstract
Genotoxicity studies have frequently been conducted on humans to evaluate the mutagenic potential associated with acute or chronic exposure to chemical agents. The more common approach involves indirect measurement of the effect of chemical agents by assessing chromosomal damage in surrogate cells, such as peripheral blood lymphocytes. From the genetic point of view, particular interest in this paper is presented the chromosomal mutagenic effect, which we can analyze cytologicily. In this cytogenetic monitoring studies the interest is chronic exposure of organism to chemical hazards (Pb, Zn and Cn) like potentially genotoxic agents. We used peripheral blood lymphocytes as the target material. The total share of structural chromosome aberration (SCA) are search out over the 3 years of monitoring on human population exposed to heavy metals. Also we measured the blood level of Pb, Zn and Cd. Chromosomal aberrations (like dicentric and acentric chromosome) were found in 7% of subjects. Also in the individuals with chromosomal aberrations we evaluated positive correlation between blood levels of heavy metals (Pb, Zn and Cd) and chromosomal aberrations. All confirmed chromosome type aberrations in the subjects were accompanied with anemia, leucocitosis and anisocitosis. From a practical point of view, the cytological assesment or monitoring which requires only a few cells (peripheral lymphocytes) and results can be obtained within a matter of hours would be suitable for use in environmental research,. Studying the effects of environmental impact on exposures intends to quantify the agents present in the environment, to investigate their effect on chronic disease occurrence in exposed individuals, also can be used in the management of cancer or any other disease. Early detection of hazardous exposures of humans may significantly reduce adverse effects through appropriate reductions in the presence of those chemicals in the general environment.
Key words: Envoriment, exposure, lymphocytes, chromosomal aberration, effects
| Item Type: | Conference or Workshop Item (Poster) |
|---|---|
| Subjects: | Medical and Health Sciences > Basic medicine |
| Divisions: | Faculty of Medical Science |
| Depositing User: | Nevenka Velickova |
| Date Deposited: | 28 Nov 2016 09:41 |
| Last Modified: | 28 Nov 2016 09:41 |
| URI: | https://eprints.ugd.edu.mk/id/eprint/16668 |
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