Georgievski, Boban and Kostik, Vesna and Stojanovska - Geprgievska, Lihnida and Kochubovski, Mihail and Memeti, Shaban (2016) Qualitative and quantitative analysis of aflatoxins in raw peanuts(Arachis hypogaea L.). Journal of Environmental Protection and Ecology (JEPE), 17 (3). pp. 961-969. ISSN 1311-5065
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Abstract
Aflatoxins are toxic cancerogenic secondary metabolites, predominantly produced by
two fungal spices: Aspergillus flavus and Aspergillus parasiticus. The toxicity of aflatoxins make them primary health hazard because of their occurrence in agricultural food crops. In order to detect presence of contaminants, usually occurring in very low concentrations of several μg/kg, precise analytical methods for detection and quantification are necessary, that have to be simple to carry out and specific, but mainly very sensitive. The subject of this paper is qualitative and quantitative
analysis of total aflatoxines (AFB1 + AFB2 + AFG1 + AFG2) in raw peanuts. The analysis has covered 78 samples of raw peanuts. Preliminary qualitative assessment indicating the presence of contaminants was performed using the method of thin layer chromatography, characterised by its simplicity, rapidity, but still sufficient accuracy to separate the contaminated samples. For quantification of aflatoxines, we developed a method of fluorometric spectroscopy. It is an effective procedure for quantification with analytical yield in the range 89.79–104.78%, correlation factor R2 = 0.9993 and sufficiently low value of the limit of quantification LoQ of 2.1 mg/kg. The analysis detected 23% of samples with concentrations of total aflatoxines above the permitted MRL, with average value of 2.6 mg/kg and the maximum of 12.8 mg/kg.
Keywords: aflatoxines, raw peanuts, TLC, fluorometry
| Item Type: | Article |
|---|---|
| Subjects: | Natural sciences > Chemical sciences Agricultural Sciences > Other agricultural sciences |
| Divisions: | Faculty of Medical Science |
| Depositing User: | Vesna Kostik |
| Date Deposited: | 28 Oct 2016 09:30 |
| Last Modified: | 28 Oct 2016 09:30 |
| URI: | https://eprints.ugd.edu.mk/id/eprint/16548 |
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