Evaluation of genotoxic variations in plant model systems in a case of metal stressors

Gjorgieva Ackova, Darinka and Kadifkova-Panovska, Tatjana and Baceva, Katerina and Stafilov, Trajče (2016) Evaluation of genotoxic variations in plant model systems in a case of metal stressors. Journal of Environmental Science and Health, Part B, 51 (5). pp. 340-349. ISSN 0360-1234 (Print), 1532-4109 (Online)

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Abstract

This study was designed to assess the effects of long term, high metal exposition (cadmium, lead, copper,
nickel and zinc) on DNA damage in four plant model systems [Taraxacum officinale (Asteraceae), Matricaria
recutita L. (Asteraceae), Robinia pseudoacacia L. (Fabaceae), and Urtica dioica (Urticaceae)]. DNA stability
was investigated by a Random Amplified Polymorphic DNA (RAPD) technique. Agarose-gel electrophoresis
revealed total of 37 bands with different molecular weights ranging from 1250 to 5000 bp. It generated
distinctive polymorphism value of 72.97% (27 bands) total in four plant species investigated. The
dendrogram constructed using NTSYSpc programme showed that there is grouping in separate clusters of
the same plant model collected from two different areas (metal-exposed and control samples). The study
concluded that the long term metal-exposing periods had genotoxic stress on macromolecules of plant
model systems investigated and biomarkers used should be augmented for reliable estimates of
genotoxicity after exposure of plants to metal stressors.

Item Type:	Article
Subjects:	Medical and Health Sciences > Other medical sciences
Divisions:	Faculty of Medical Science
Depositing User:	Darinka Gorgieva Ackova
Date Deposited:	07 Jul 2016 10:12
Last Modified:	07 Jul 2016 10:12
URI:	https://eprints.ugd.edu.mk/id/eprint/15951

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