Velickova, Nevenka and Milev, Mishko and Nedeljkovik, Bojana and Gorgieva, Pale (2015) Practices the different cells types like target genotoxic endpoint in micronucleus assey. In: The 1th Conference of Medical and Biological Engineering in Bosnia and Herzegovina (CMBEBIH 2015), 13-15 March 2015, Sarajevo, BiH.
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Abstract
Introduction: The toxicological relevance of the micronucleus (MN) assey is well defined: it is a multi-target genotoxic endpoint, assessing not only clastogenic and aneugenic events but also some epigenetic effects, which is simple to score, accurate, applicable in different cell types. Scoring of micronuclei can be performed relatively easily and on different cell types relevant for human biomonitoring: lymphocytes, fibroblasts and exfoliated epithelial cells. Aims of the study: To indicate the importance of the application of micronucleus test as standardized cytogenetic method as an important biomarker in detecting the impact of ionizing radiation on the entire genetic material in occupationally exposed health care workers. Matherial and methods: The study include health professionals who are directly and on daily basses exposed to ionizing radiation as physical agents and a control group that represents young and healthy population that is not exposed to any physical and chemical agents. Results. The major advantage of lymphocytes is that they are primary cells, easy to culture in suspension. The choice between whole blood and isolated lymphocytes depends upon the question addressed. The most important differences among the protocols are the hypotonic treatment (critical in particular for image analysis), fixation of the cells (dependent on laboratory preferences) and the final slide preparation. Conclusion: In recent years the in vitro micronucleus test has become an attractive tool for genotoxicity testing because of its simplicity of scoring and wide applicability in different cell types but the lymphocytes are the most reperesentative cells for this kind of research.
Keywords: cells cultures, lymphocytes, micronucleus, genotoxicology, radiation
| Item Type: | Conference or Workshop Item (Lecture) |
|---|---|
| Subjects: | Medical and Health Sciences > Basic medicine |
| Divisions: | Faculty of Medical Science |
| Depositing User: | Nevenka Velickova |
| Date Deposited: | 23 Dec 2015 13:06 |
| Last Modified: | 23 Dec 2015 13:06 |
| URI: | https://eprints.ugd.edu.mk/id/eprint/14670 |
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