Detection of circulating proteins and markers of oxygen deficit

Domazetovska, Saska and Vavlukis, Marija and Petrovska, Emilija and Biljali, Sefedin and Janikevik Ivanovska, Danijela and Kuzmanovska, Sonja and Grozdanovska Naumovska, Mirjana (2014) Detection of circulating proteins and markers of oxygen deficit. In: IFCC Worldlab Istanbul 2014, 22-26 June 2014, Istanbul, Turkey.

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BACKGROUND: Persistent quest for cell response to a stress provoked by different origin, leads to investigation of models which can assure enough date. Heart tissue ischemic events with high prevalence of morbidity is one of the most research area. So far, troponin, along with CK-MB provides reliable information in diagnosis of acute myocardial infarction. Introduction of heat shock proteins (HSP70) as molecular chaperons of processes which prevent further cell integrity destruction, shows better view to a situation. This study was aimed to distinguish diagnosis and presuppose
prognosis at patients admitted to emergency room (ER) with symptoms for acute coronary syndrome (ACS).
METHODS: Beside usually used parameters, samples of 200 patients were tested for troponins, myoglobin and HSP
70. Troponin and myoglobin were detiminate with ECLIA (ElectroChemiLuminiscenceImmunoAssay - Elecsys 2010)
and HSP70 with ELISA (EnzymeLinkImmunoSorbentAssay) method. Estimated results for parameters determinate at
patients from ER were statistically compared to results received by testing samples from healthy blood donors as
control group (C).
RESULTS: Presence of oxygen stress was confirm as elevated level of HSP 70 at patients diagnosed with AIM (26,3 times-fold vs. C) and unstable angina (15,1 times- fold vs. C). Those results implicate that during this condition, cell engage mechanisms and processes for cell integrity protection and its components. In our opinion this is also confirm with elevated reactive proteins level (8,5 times- fold vs C) and its regulation in a few weeks, if there is not any adverse events. In other experiment this can be supported by measuring number or level of cell involved in immune-response and production of specific antibodies which can help laboratory workers and physicians in modeling the protocols.
CONCLUSIONS: The results of this study fulfill expectation in diagnostic and prognostic value of HSP70 and further treatment of patients suffering ACS. Due to long lasting procedure and compare to results received for the troponins, our suggestions goes to use of specific proteins as diagnostic parameters at very first moment. HSP determination has its importance in follow up of the patients suffering ACS

Item Type: Conference or Workshop Item (Poster)
Additional Information: Code (0332 S521)
Subjects: Medical and Health Sciences > Basic medicine
Medical and Health Sciences > Clinical medicine
Medical and Health Sciences > Health sciences
Medical and Health Sciences > Other medical sciences
Divisions: Faculty of Medical Science
Depositing User: Danijela Janikevik
Date Deposited: 21 Dec 2015 14:28
Last Modified: 12 Apr 2016 09:55

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