Validation of biomarkers of oxidative stress in large-scale human studies

Jansen, Eugene and Beekhof, Piet and Viezeliene, Dale and Ruskovska, Tatjana and Muzakova, Vladimira and Skalicky, Jiri (2015) Validation of biomarkers of oxidative stress in large-scale human studies. In: 15th International Nutrition & Diagnostics Conference INDC 2015, 5-8 Oct 2015, Prague, Czech Republic.

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Objectives. Oxidative stress has been proposed to be important in age-related processes and chronic
diseases. Before biomarkers of oxidative stress are measured in large-scale human studies, they need
validation on storage stability and other characteristics. Amongst several candidates (MDA, FRAP,
TAS, TOS, GSH, GPX, SOD), three biomarkers were selected which cover different aspects of the
oxidative stress process.
Methodology. ROM (Reactive Oxygen Metabolites, Diacron) was used as biomarker of oxidative
stress, BAP (Biological Antioxidant Potential, Diacron) for anti-oxidant status and TTL (Total Thiol
Levels, RelAssay) for the redox status. (DX, Beckman-Coulter).
Results. The assays for the biomarkers ROM, BAP and TTL were adapted for an auto-analyzer (DX,
Beckman-Coulter) with very good reproducibility. The biomarkers were stable on short- and long-term
storage. Also they showed no significant differences in blood samplings at different times of the day,
not a post-prandial effect. This set of biomarkers was successfully applied in several large-scale
European studies (total 20,000 samples) on aging and nutrition.
Conclusion. A set of three biomarkers of oxidative stress was selected for use on an auto-analyzer
and applied successfully in large-scale European studies.

Item Type: Conference or Workshop Item (Poster)
Subjects: Medical and Health Sciences > Clinical medicine
Medical and Health Sciences > Health sciences
Divisions: Faculty of Medical Science
Depositing User: Tatjana Ruskovska
Date Deposited: 27 Oct 2015 14:04
Last Modified: 27 Oct 2015 14:04

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