HPV E6/E7 mRNA Versus HPV DNA Biomarker in Cervical Cancer Screening of a Group of Macedonian Women

Duvlis, Sotirija and Popovska-Jankovic, Katerina and Arsova-Sarafinovska, Zorica and Memeti, Shaban and Popeska, Zaneta and Plaseska-Karanfilska, Dijana (2015) HPV E6/E7 mRNA Versus HPV DNA Biomarker in Cervical Cancer Screening of a Group of Macedonian Women. Journal of Medical Virology. ISSN 1096-9071

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High risk types of human papillomaviruses E6/E7 oncogenes and their association with tumor suppressor genes products are the key factors of cervical carcinogenesis. This study proposed them as specific markers for cervical dysplasia screening. The aim of the study is to compare the clinical and prognostic significance of HPV E6/E7 mRNA as an early biomarker versus HPV DNA detection and cytology in triage of woman for cervical cancer. The study group consists of 413 women: 258 NILM, 26 ASC-US, 81 LSIL, 41 HSIL, and 7 unsatisfactory cytology. HPV4AACE screening, real-time multiplex PCR and MY09/11 consensus PCR primers methods were used for the HPV DNA detection. The real-time multiplex nucleic acid sequence-based assay (NucliSENS EasyQ HPV assay) was used for HPV E6/E7 mRNA detection of the five most common high risk HPV types in cervical cancer (16, 18, 31, 33, and 45). The results show that HPV E6/E7 mRNA testing had a higher specificity 50% (95% CI 32–67) and positive predictive value (PPV) 62% (95% CI 46–76) for CIN2+ compared to HPV DNA testing that had specificity of 18% (95% CI 7–37) and PPV 52% (95% CI 39–76) respectively. The higher specificity and PPV of HPV E6/E7 mRNA testing are valuable in predicting insignificant HPV DNA infection among cases with borderline cytological finding. It can help in avoiding aggressive procedures (biopsies and over-referral of transient HPV infections) as well as lowering patient's anxiety and follow up period.

Item Type: Article
Subjects: Medical and Health Sciences > Basic medicine
Natural sciences > Biological sciences
Medical and Health Sciences > Clinical medicine
Medical and Health Sciences > Health sciences
Divisions: Faculty of Medical Science
Depositing User: Zorica Arsova Sarafinovska
Date Deposited: 03 Jun 2015 07:55
Last Modified: 03 Jun 2015 07:55
URI: https://eprints.ugd.edu.mk/id/eprint/13248

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