Arsova-Sarafinovska, Zorica and Aydin, Ahmet and Sayal, Ahmet and Eken, Ayse and Erdem, Onur and Savaser, Ayhan and Erten, Koray and Ozgok, Yasar (2007) Rapid and Simple Determination of Plasma and Erythrocyte MDA Levels in Prostate Cancer Patients by a Validated HPLC Method. Journal of Liquid Chromatography & Related Technologies, 30. pp. 2435-2444. ISSN 1082-6076 print/1520-572X online
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Rapid and Simple Determination of Plasma and Erythrocyte MDA Levels in Prostate Cancer Patients by a Validated HPLC Method.pdf Download (79kB) | Preview |
Abstract
We undertook the present study to develop a validated HPLC method for the determination of malondialdehyde (MDA) levels and to use this method for determination of MDA levels in patients with prostate cancer and benign prostatic hyperplasia. MDA levels were estimated in the erythrocyte and plasma sample of the 25 non-metastatic prostate cancer patients, 36 benign prostatic hyperplasia (BPH) patients and 24 age- and sex-matched healthy subjects (controls) in HP Chromatographic systems consisting of a Model Agilent 1100 Series. We report a very rapid and simple isocratic reversed-phase HPLC separation of MDA in normal human plasma and erythrocytes without previous purification of theMDA-TBA complex. All validation parameters were performed in our methods. Using this methods we have found elevated MDA in the plasma and erythrocyte of the prostate cancer group as compared to controls and BPH group. We have improved and validated an analytical HPLC method for determination of MDA in plasma and erythrocyte, which is simple to perform and having high sensitivity, specificity and substantial improvement in column life. This method has been successfully applied to determination of MDA levels in prostate cancer patients and offers an oportunity to further characterize the role of oxidative injury in the pathogenesis of this disease
specifically.
Keywords: Prostate cancer, Oxidative stress, Lipid peroxidation, HPLC
| Item Type: | Article |
|---|---|
| Subjects: | Medical and Health Sciences > Basic medicine Natural sciences > Biological sciences Natural sciences > Chemical sciences Medical and Health Sciences > Clinical medicine Medical and Health Sciences > Health sciences |
| Divisions: | Faculty of Medical Science |
| Depositing User: | Zorica Arsova Sarafinovska |
| Date Deposited: | 01 Jun 2015 08:10 |
| Last Modified: | 11 Nov 2015 14:48 |
| URI: | https://eprints.ugd.edu.mk/id/eprint/13222 |
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