Kostik, Vesna and Bauer, Biljana (2015) Antifungal activity of the essential oils of wild-growing Mentha piperita L and Mentha spicata L from the Mariovo region, Republic of Macedonia. In: The Second Mediterranean Symposium on Medicinal and Aromatic Plants (MESMAP-2), 22-25 Apr 2015, Antalya, Turkey.
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Abstract
The antifungal activity of the essential oils isolated by hydro-distillation from the leaves of wild growing Mentha piperita and Mentha spicata (Lamiaceae) at the region of Mariovo, Republic of Macedonia was tested by disc diffusion method and the micro-dilution broth method (MIC) against six plant pathogenic fungi: Alternaria alternate Alternaria solani, Aspergillus flavus, Aspergillus niger, Fusarium solani and Rhizopus solani. The results from the disc diffusion method followed by MIC indicated that M. spicata essential oil showed maximum antifungal activity with larger inhibition zone (20 – 29 mm) and smallest MIC values (65.8 – 120.3 μg mL-1) against all the strains tested. M. piperita essential oil exhibited good antifungal activity with inhibition zone of 19 and 20 mm and MIC values of 120.3 and 115.4 μg mL- 1, respectively against Fusarium solani and Aspergilus flavus and excellent antifungal activity with inhibition zone of 28 and 30 mm and MIC values of 65.4 and 50.6 μg mL- 1, respectively against Aspergilus niger and Rhizopus solani.
Menthol, menthone, carvone, limonene and 1,8 - cineole, the major constituents of M. piperita and M. spicata, were also tested for their potential antifungal activity. Posaconazole a triazole antifungal drug was used as a positive reference drug for fungi. Menthol exhibited excellent antifungal activity against Alternaria solani, Alternaria alternata, Aspergilus niger and Rhizopus solani (inhibition zone 23 – 31 mm, MIC 36.4 – 76.8 μg mL-1), which was close to the antifungal activity of standard drug for the strains Alternaria alternata, Alternaria solani and Rhizopus solani. Menthone and carvone showed good antifungal activity against Aspergilus flavus, Aspergilus niger and Rhizopus solani (inhibition zone 20 – 29 mm, MIC 58.3 – 114.5 μg mL-1). Carvone exhibited better antifungal activity for Fusarium solani than menthone. Contrary to these findings limonene and 1,8-cineol exhibited lower antifungal activity for all tested strains (inhibition zone 10-15 mm, MIC 69.3 – 220.6 μg mL-1.
Keywords: Mentha, essential oils, fungi, posaconazole
| Item Type: | Conference or Workshop Item (Poster) |
|---|---|
| Subjects: | Medical and Health Sciences > Other medical sciences |
| Divisions: | Faculty of Medical Science |
| Depositing User: | Vesna Kostik |
| Date Deposited: | 04 May 2015 09:06 |
| Last Modified: | 07 Aug 2015 09:56 |
| URI: | https://eprints.ugd.edu.mk/id/eprint/13113 |
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