Mechanism in the Era of Transporter Structures

Pereira, Carlos M. and Gulaboski, Rubin and Silva, A. Fernando (2005) Mechanism in the Era of Transporter Structures. In: Conference of Electrochemistry-Porto, 5-9 April 2005, Porto, Portugal.

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The properties of the interface between the water and DCE has some similarities with the
interface between the biological membranes and water.[1] In order to increase the degree of
similarity the water/DCE interface is modified with various lipids.[2,3] In this way, the ITIES
mimics closely the bio-membranes,[4] and, without a doubt, it is possible to extract more
reliable information about the processes taking place in the living organisms. Recently a
particular interest has been devoted to the ion transfer across the interface of two immiscible
solvents since these systems can be used to better understand the mechanisms involved in the
process of drug uptake.
In this work we will present experimental results obtained in the study of the transfer of
acetylcholine cations across the interface of water and 1,2-dichloroethane-modified by
dioleoylacetylcholine phosphatidylcholine (DOPC) and dipalmitoylacetylcholine
phosphatidylcholine (DPPC). Phosphatidylcholine were chosen since they are an important
component of cellular membranes and they are also the major delivery form of the essential
nutrient choline which in turn is itself a precursor in the synthesis of the neurotransmitter
The experimental techniques used in this work include electrochemical techniques
(voltammetry and electrochemical impedance) as well as Langmuir compression isotherms.
From the experimental data it is possible to evaluate the kinetic parameters of the ion transfer
and an interaction constant between the lipid and the acetylcholine cation and the results will
be presented and discussed.

Item Type: Conference or Workshop Item (Speech)
Subjects: Natural sciences > Biological sciences
Natural sciences > Chemical sciences
Natural sciences > Physical sciences
Divisions: Faculty of Agriculture
Depositing User: Rubin Gulaboski
Date Deposited: 18 Nov 2012 21:30
Last Modified: 18 Nov 2012 21:30

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