Optimization of the formulation for preparing Lactobacillus casei loaded whey protein-Ca-alginate microparticles using full-factorial design

Smilkov, Katarina and Petreska Ivanovska, Tanja and Petrusevska Tozi, Lidija and Petkovska, Rumenka and Hadzieva, Jasmina and Popovski, Emil and Stafilov, Trajče and Grozdanov, Anita and Mladenovska, Kristina (2013) Optimization of the formulation for preparing Lactobacillus casei loaded whey protein-Ca-alginate microparticles using full-factorial design. Journal of Microencapsulation. ISSN 0265-2048 (print), 1464-5246 (online)

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Official URL: http://informahealthcare.com/doi/abs/10.3109/02652...

Abstract

Context: This article presents specific approach for microencapsulation of Lactobacillus casei using emulsion method followed by additional coating with whey protein. Methods: Experimental design was employed using polynomial regression model at 2nd level with three independent variables, concentrations of alginate, whey protein and CaCl2. Physicochemical, biopharmaceutical and biological properties were investigated. Results: In 11 series generated, negatively charged microparticles were obtained, with size 6.99–9.88 mm, Ca-content 0.29–0.47mg per 10mg microparticles, and viability of the probiotic 9.30–10.87 log10CFU/g. The viability after 24 hours in simulated gastrointestinal conditions was between 3.60 and 8.32 log10CFU/g. Discussion: Optimal formulation of the microparticles that ensures survival of the probiotic and achieves controlled delivery was determined: 2.5% (w/w) alginate, 3% (w/w) CaCl2 and 3% (w/w) whey protein. Conclusion: The advantageous properties of the L. casei-loaded microparticles make them suitable for incorporation in functional food and/or pharmaceutical products.

Item Type: Article
Subjects: Natural sciences > Biological sciences
Natural sciences > Chemical sciences
Medical and Health Sciences > Health biotechnology
Medical and Health Sciences > Health sciences
Divisions: Faculty of Medical Science
Depositing User: Katarina Smilkov
Date Deposited: 25 Nov 2013 15:03
Last Modified: 27 Oct 2014 11:30
URI: http://eprints.ugd.edu.mk/id/eprint/7144

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