Rapid MALDI-TOF-MS Detection of Anthocyanins in Wine and Grape Using Different Matrices

Ivanova, Violeta and Dörnyei, Ágnes and Stefova, Marina and Stafilov, Trajče and Vojnoski, Borimir and Kilár, Ferenc and Márk, László (2011) Rapid MALDI-TOF-MS Detection of Anthocyanins in Wine and Grape Using Different Matrices. Food Analytical Methods, 4. pp. 108-115.

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Abstract

Matrix-assisted laser desorption/ionization timeof- flight mass spectrometry (MALDI-TOF-MS) as a new valuable technique has been used for qualitative screening of anthocyanins in wine and grape samples from Vranec and Merlot variety. For that purpose, different MALDI matrices have been tested, such as α-cyano-4-hydroxycinnamic acid, sinapic acid, 2,5-dihydroxybenzoic acid (2,5- DHB), and C70 fullerene. It was observed that 2,5-DHB was superior with respect to all the matrices used in the study. Furthermore, fullerene was, for the first time, tested and used as a possible matrix for MALDI-TOF-MS identification of anthocyanins in wine and grape samples. The results obtained showed identification of aglycone forms of anthocyanins only, since the higher laser energy applied for ionization of the fullerene molecules led to great deal of fragmentation of the sugar moiety from glucosides, acetylglucoside, and p-coumaroylglucoside groups from the corresponding anthocyanin molecules. Additionally, liquid chromatography/electrospray ionization mass spectrometry has also been applied for anthocyanin characterization.

Item Type: Article
Subjects: Agricultural Sciences > Agricultural biotechnology
Natural sciences > Chemical sciences
Divisions: Faculty of Agriculture
Depositing User: Violeta Ivanova Petropulos
Date Deposited: 04 Nov 2012 17:50
Last Modified: 28 Oct 2013 12:39
URI: http://eprints.ugd.edu.mk/id/eprint/305

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