Antimicrobial activity of pomegranate peel extracts from "Karamustafa" and "Idzis" varieties

Dimovska, Violeta and Ilieva, Fidanka and Mihajlov, Ljupco and Kostadinovic Velickovska, Sanja and Kovacevik, Biljana (2018) Antimicrobial activity of pomegranate peel extracts from "Karamustafa" and "Idzis" varieties. In: 1st International Meeting Agriscience & Practice (ASP 2018), 10-11 May 2018, Stip, Macedonia.

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Abstract

The aim of this study was determination of antimicrobial activity of pomegranate peel extracts from “Karamustafa” and “Idžis” varieties. For this purposes, the peel of the two varieties of pomegranates was dried, powdered and extracted three times by methanol: water solution (80:20). After evaporation, the extracts were investigated for their “in vitro” antibacterial and antifungal properties using a disk-diffusion method in Petri dishes. The pomegranate peel extracts were tested for antibacterial activity against one Gram-positive bacterial strain Staphylococcus aureus (ATCC 25923), and against one Gram-negative bacterial strain Escherichia coli (ATCC 25922), and for antifungal activity using Candida albicans (ATCC 1023). In brief, each microorganism was suspended in Mueller Hinton (MH) broth and diluted approximately to 10E6 colony forming unit (cfu)/mL. Gentamicin (20 μg/well), nalidixic acid (30 μg/well), ciprofloxacine (5 μg/well) and erytromicine (15 μg/well) were used as positive control. The antibacterial activity is ranked from no activity (-: inhibition diameter < 10 mm), low (+: inhibition diameter between 10 and 15 mm), moderate (++: inhibition diameter between 15 and 20 mm) and high activity (+++: diameter inhibition ≥ 20 mm). All tests were performed in triplicate and clear halos greater than 10 mm were considered as positive results. Our results showed very high antimicrobial activity of the extracts from both variety against Staphylococcus aureus (ATCC 25923) with inhibition zone of 40 mm. The antifungal activity against Candida albicans (ATCC 1023) was very low with inhibition zone of 10 mm.

Item Type: Conference or Workshop Item (Poster)
Subjects: Agricultural Sciences > Agricultural biotechnology
Agricultural Sciences > Other agricultural sciences
Divisions: Faculty of Agriculture
Depositing User: Sanja Kostadinovik
Date Deposited: 29 May 2018 11:37
Last Modified: 29 May 2018 11:37
URI: http://eprints.ugd.edu.mk/id/eprint/20022

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