Multigene characterization of ‘Candidatus phytoplasma solani’ in pepper and tomato plants in the Republic of Macedonia

Arsov, Emilija and Ivanova, Galina and Mitrev, Sasa (2017) Multigene characterization of ‘Candidatus phytoplasma solani’ in pepper and tomato plants in the Republic of Macedonia. Journal of Agriculture and Plant Sciences, 15 (1/2). pp. 7-15. ISSN 2545-4455

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Abstract

‘Candidatus Phytoplasma solani’ is a phytoplasma distributed worldwide, associated in the Euro-Mediterranean area with various diseases in most spreader and cultivated plants including grapevine, maize, potato, pepper, celery and tobacco. Molecular multigene characterization was performed for the first time on phytoplasma strains collected from the Eastern part of Macedonia from symptomatic and asymptomatic pepper and tomato plants, during the summer period of the 2015/16 season. PCR amplification on specific tuf, vmp1 and stamp genes showed that the phytoplasma strains identified belong to subgroup 16SrXII-A, since their restriction patterns were indistinguishable from one another and from the patterns characteristic of the STOL (16SrXII-A) reference strain. For samples positive for the tuf gene, it was possible to identify the HpaII RFLP profiles associated with tuf type b genes. This is the first field observation and molecular laboratory testing for the presence of stolbur phytoplasma in pepper and tomato in the Republic of Macedonia.

Item Type: Article
Subjects: Agricultural Sciences > Agricultural biotechnology
Divisions: Faculty of Agriculture
Depositing User: Emilija Arsov
Date Deposited: 26 Dec 2017 13:31
Last Modified: 26 Dec 2017 13:31
URI: http://eprints.ugd.edu.mk/id/eprint/18844

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