A Simple Method for Determination of Protodioscin in Tribulus Terrestris L. and Pharmaceuticals by High-Performance Liquid Chromatography Using Diode-Array Detection

Shishovska, Maja and Arsova-Sarafinovska, Zorica and Memeti, Shaban (2015) A Simple Method for Determination of Protodioscin in Tribulus Terrestris L. and Pharmaceuticals by High-Performance Liquid Chromatography Using Diode-Array Detection. Journal of Chemical Engineering Research Updates, 2. pp. 12-21. ISSN 2409-983X/15 (In Press)

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Abstract

The Tribulus terrestris L. (Zygophyllaceae) is a known medical plant used in traditional and folk medicine
worldwide. The steroid saponine protodioscin, an active component found in this plant, serves as a marker for quality
control of plant raw materials. In this study, we developed a simple and selective method for determination of
protodioscin using convenient High-Performance Liquid Chromatographic (HPLC) laboratory equipment. The detection
was performed by Diode-Array Detector (DAD). The proposed method was fully validated and according to the validation
results, it was accurate and precise. It was proven to be linear over a protodioscin concentration range of 10,9 to 544.9
μg/mL. The low values of limit of detection (LOD) and limit of quantitation (LOQ) demonstrated adequate sensitivity (16.0
μg and 48.6 μg protodioscin per g plant material, respectively). The proposed method was successfully applied for
quality control of a raw plant material intended for use in pharmaceutical industry, as well as for determination of
protodioscin in a commercially available pharmaceutical formulation. The positive identification of protodioscin in
analyzed samples was done by comparison of retention times of chromatographic peaks and their UV spectra. The
content of protodioscin in analyzed samples was: 0.65 – 0.73 % in a raw plant material, and 0.38 % in tablets,
respectively.

Item Type: Article
Subjects: Medical and Health Sciences > Basic medicine
Natural sciences > Biological sciences
Medical and Health Sciences > Other medical sciences
Divisions: Faculty of Medical Science
Depositing User: Zorica Arsova Sarafinovska
Date Deposited: 08 May 2015 11:27
Last Modified: 08 May 2015 11:27
URI: https://eprints.ugd.edu.mk/id/eprint/13129

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