Application of a Novel Small-Scale Sample Cleanup Procedure Prior to MALDI-TOF-MS for Rapid Pigment Fingerprinting of Red Wines

Ivanova, Violeta and Dörnyei, Ágnes and Stefova, Marina and Stafilov, Trajče and Vojnoski, Borimir and Márk, László and Hermosín-Gutiérrez, Isidro and Kilár, Ferenc (2014) Application of a Novel Small-Scale Sample Cleanup Procedure Prior to MALDI-TOF-MS for Rapid Pigment Fingerprinting of Red Wines. Food Analytical Methods, 7 (4). pp. 820-827. ISSN 1936-9751

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Official URL: http://link.springer.com/article/10.1007/s12161-01...

Abstract

This study evaluates the anthocyanin and derived pigment composition of Vitis vinifera red wines of Vranec, Merlot, and Cabernet Sauvignon produced in 2006, 2007, and 2008 vintages from the Tikve š wine region in the Republic of Macedonia.Their profile was established using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) technique. A total of 22 anthocyanins and derived pigments have been identified in the samples including 10 anthocyanins, 1 ethyl-bridged flavanol – anthocy- anin adduct, and 11 pyranoanthocyanins. MALDI-TOF-MS analysis was performed after solid-phase extraction of the wines by using, for the first time, the Zip-Tip® C18 stationary phase, introducing a novel small-scale sample cleanup proce- dure prior to the rapid MALDI-TOF-MS fingerprinting of wine samples. 2 ′ ,4 ′ ,6 ′ -Trihydroxyacetophenone (dissolved in acetonitrile/water 1:1, v / v ) was used as a matrix. The qualitative screening of anthocyanins and derived pigments with MALDI- TOF-MS confirmed the presence of glucoside, acetylglucoside, and p -coumaroylglucoside derivatives of anthocyanins in the wine samples. Furthermore, pyranoanthocyanins formed by reactions of anthocyanins with pyruvic acid and acetaldehyde, as well as flavanol – pyranoanthocyanins and ethyl-bridged flavan-3-ol-anthocyanin adduct pigments have been detected in the samples.

Item Type: Article
Subjects: Agricultural Sciences > Agricultural biotechnology
Natural sciences > Chemical sciences
Divisions: Faculty of Agriculture
Depositing User: Violeta Ivanova Petropulos
Date Deposited: 04 Mar 2014 12:10
Last Modified: 04 Mar 2014 12:10
URI: http://eprints.ugd.edu.mk/id/eprint/9354

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