Preparing the dental pulp for histopathological investigation

Georgiev, Zlatko and Kovacevska, Ivona and Dimova, Cena (2013) Preparing the dental pulp for histopathological investigation. In: 18th Congress of the Balkan Stomatological Society (BaSS), 25-28 Apr 2013, Skopje, Macedonia.

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The aim of our study was to evaluate the condition, particular structure and properties of the pulpal tissue by using various methods.The pulps used for this research had originated from intact teeth of healthy children, (five deciduous teeth without, and five deciduous teeth with physiological resorption).Immediately after the extraction (performed due to orthodontic reasons, under local anesthesia), each tooth was cut perpendicularly to its long axis with a rotating carborundum disc under a water jet. The separated halves were dissected with plastic instrument, and the tooth pulp was excavated completely The extracellular matrix is the major constituent of the connective tissue. This is composed of ground substance and fibrillar proteins. The main cells of the connective tissue are the fibroblasts. The pulp also contains odontoblasts (the highest differentiated cells), undifferentiated mesenchyme cells, and immunocompetent cells (lymphocytes, macrophages, leucocytes).Adequate pulp preparation has always been a challenge, because artefacts resulting from inadequate fixation often are described as evidence of pathosis.Methods with dropping a tooth in a jar of formalin, even if done immediately after extraction, are inadequate to permit subsequent critical examination of the dental pulp. Other methods are with section the apical 2–3 mm of the root with a fissure bur, and an opening was made into the pulp chamber with a round bur and fixed with 10% buffered formalin.The developments of models are needed for better investigation of deciduous dental pulp, but also for better her preservation

Item Type: Conference or Workshop Item (Speech)
Subjects: Medical and Health Sciences > Basic medicine
Medical and Health Sciences > Clinical medicine
Medical and Health Sciences > Other medical sciences
Divisions: Faculty of Medical Science
Depositing User: Cena Dimova
Date Deposited: 25 Nov 2013 10:09
Last Modified: 19 Feb 2014 08:58

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