Generation of induced pluripotent stem cells from patient-specific FSHD myoblasts

Bosnakovski, Darko and Nandez, Ramiro and Struck, Matthew and Zaidman, Nathan and Kyba, Michael (2009) Generation of induced pluripotent stem cells from patient-specific FSHD myoblasts. In: 13th Annual Minnesota Muscle Symposium, 9 June 2009, USA.

[img]
Preview
Text
MN-Muscle-AbstractBook2009.pdf

Download (1497Kb) | Preview

Abstract

Generation of induced pluripotent stem cells from patient-specific FSHD myoblasts Ramiro Nandez, Darko Bosnakovski, Mathew Struck, Nathan Zaidman and Michael Kyba Lillehei Heart Institute and Department of Pediatrics, University of Minnesota Facioscapulohumeral muscular dystrophy (FSHD) is a dominant inherited neuromuscular disease. The underlying molecular mechanism of the disease is still unknown but 95% of patients carry a subtelomeric deletion on chromosome 4q35.2 thought to affect chromatin and gene expression in the region. The recent discovery of methods to reprogram somatic cells to the embryonic state allows the creation of patient- and disease-specific cells, which can serve as a model for the disease, and the possibility of autologous cell therapy with genenetically corrected cells. In this study we have generated 3 induced pluripotent stem (iPS) cell lines from myoblasts obtained from FSHD patient biopsies as well as a 4th line derived from unaffected myoblasts. To induce reprogramming we delivered the classical transcription factor quartet (Oct4, Sox2, Klf4, and Myc) by retroviral transduction and also included SV40 Large-T-antigen to increase the efficiency of reprogramming. These cell lines appear indistinguishable from human embryonic stem (hES) cells and express hES markers. We are in the process of characterizing each of the clones for gene expression, teratoma formation and in vitro differentiation with the intention of understan

Item Type: Conference or Workshop Item (Speech)
Subjects: Natural sciences > Biological sciences
Divisions: Faculty of Medical Science
Depositing User: Darko Bosnakovski
Date Deposited: 24 Jan 2014 11:56
Last Modified: 24 Jan 2014 11:56
URI: http://eprints.ugd.edu.mk/id/eprint/7052

Actions (login required)

View Item View Item