Maintenance of Human iPS Cells in a Feeder-free Culture System

Bosnakovski, Darko and Islam, Shabana and Nandez, Ramiro and Zaidman, Nathan and Struck, Matthew and Dandapat, Abhijit and Kyba, Michael (2012) Maintenance of Human iPS Cells in a Feeder-free Culture System. BD Biosciences.

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The establishment of human induced pluripotent stem cells (iPSCs) has revealed a new path for the generation of stem cells without the use of embryos. This new approach eliminates ethical concerns and provides an invaluable contribution to the fi eld of regenerative medicine and stem cell biology. The direct reprogramming of somatic cells to pluripotency was fi rst accomplished in 2006, when Takahashi and Yamanaka converted adult mouse fi broblasts to iPSCs through ectopic expression of a select group of transcription factors1. Subsequent reports optimized this technique, demonstrating that regardless of the gene combination used iPSCs were highly similar to embryonic stem cells (ESCs) in their morphology, ability to express cell surface markers typical of pluripotent cells, form viable chimeras, and give rise to teratomas containing cell types representative of all three germ layers.

Item Type: Article
Subjects: Natural sciences > Biological sciences
Divisions: Faculty of Medical Science
Depositing User: Darko Bosnakovski
Date Deposited: 29 Aug 2013 11:30
Last Modified: 29 Aug 2013 11:30

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