Inducible cassette exchange: a rapid and efficient system enabling conditional gene expression in embryonic stem and primary cells.

Iacovino, Michelina and Bosnakovski, Darko and Fey, Holger and Rux, Danielle and Bajwa, Gagan and Mahen, Elisabeth and Mitanoska, Ana and Xu, Zhaohui and Kyba, Michael (2011) Inducible cassette exchange: a rapid and efficient system enabling conditional gene expression in embryonic stem and primary cells. Stem Cells, 29 (10). ISSN 1580–1588

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Official URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC362272...

Abstract

Genetic modification is critically enabling for studies addressing specification and maintenance of cell fate; however, methods for engineering modifications are inefficient. We demonstrate a rapid and efficient recombination system in which an inducible, floxed cre allele replaces itself with an incoming transgene. We target this inducible cassette exchange (ICE) allele to the (HPRT) locus and demonstrate recombination in murine embryonic stem cells (ESCs) and primary cells from derivative ICE mice. Using lentivectors, we demonstrate recombination at a randomly integrated ICE locus in human ESCs. To illustrate the utility of this system, we insert the myogenic regulator, Myf5, into the ICE locus in each platform. This enables efficient directed differentiation of mouse and human ESCs into skeletal muscle and conditional myogenic transdetermination of primary cells cultured in vitro. This versatile tool is thus well suited to gain-of-function studies probing gene function in the specification and reprogramming of cell fate.

Item Type: Article
Subjects: Natural sciences > Biological sciences
Divisions: Faculty of Medical Science
Depositing User: Darko Bosnakovski
Date Deposited: 29 Aug 2013 11:24
Last Modified: 29 Aug 2013 11:24
URI: http://eprints.ugd.edu.mk/id/eprint/7042

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