Microtechnique of 2-Mercaptoethanol test in Serologic Diagnosis of Human Brucellosis

Taleski, Vaso (1997) Microtechnique of 2-Mercaptoethanol test in Serologic Diagnosis of Human Brucellosis. In: 8th European Congress of Clinical Microbiology and Infectious Diseases, 25-28 May, 1997, Lausanne, Switzerland.

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Abstract

Microtechnique of 2-Mercaptoethanol test in Serologic Diagnosis of Human Brucellosis V.Taleski. Institute of Preventive Medical Care, Centre of Military Health Institutions, Skopje, Republic of Macedonia Objectives: A comparative assesment of Microtechnique of 2-Mercaptoethanol test (MAT 2-ME) in comparison with 2-Mercaptoethanol test (2-ME) in serologic diagnosis of human brucellosis. Methods: 2-ME was performed in tubes and MAT 2-ME in microplates. The sera were incubated at 37 C0 for 24h. Titers = or > of 160 were considered as positive. These tests confirm only IgG as agglutinable antibodies in sera. Results : A total of 223 sera were examined with both methods. Equal titers were in 212 (95%). The differences of two or more dilutions in 11 (5%) sera were statistically insignificant (p>0.005) MAT 2-ME has some advantages: need 20 fold lesser Brucella antigen, the irritation of mucoses is significantly lesser, the simply and fast use, the additional equipment is not expensive. Conclusions: MAT 2-ME is very accurate method with some advantages in comparison with 2-ME.Since the results of this test, in combination with the results of The Serum Agglutination Test-Wright, are an important indicator of the activity and stage of the disease and response to the antibiotic treatment, this test should be used as one of the routine serologic tests.

Item Type: Conference or Workshop Item (Poster)
Subjects: Medical and Health Sciences > Other medical sciences
Divisions: Faculty of Medical Science
Depositing User: Vaso Taleski
Date Deposited: 01 Feb 2013 14:31
Last Modified: 01 Feb 2013 14:31
URI: http://eprints.ugd.edu.mk/id/eprint/5394

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