Total antioxidant capacity of certain medicinal plants assesed with FRAP method and cyclic voltammetry

Maksimova, Viktorija and Ruskovska, Tatjana and Naumova, Galaba and Mirceski, Valentin and Jancovska, Maja (2012) Total antioxidant capacity of certain medicinal plants assesed with FRAP method and cyclic voltammetry. In: XXII Congress of chemists and technologists of Macedonia with international participation, 5-9.09.2012, Ohrid, R. Macedonia.

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Abstract

Antioxidants are substances that protect cells damage caused by free radicals. Many favorable biological properties, such as antimutagenic, anticarcinogenic and antiaging, among others, may originate from antioxidant property of particular substance. Plant phenolic compounds (e.g. phenolic acids, flavonoids, quinones, coumarins, lignans, stilbenes, and tannins), nitrogen compounds (e.g., alkaloids, amines), carotenoids and vitamins are the most important plant substances exhibiting antioxidant activity. The aim of this study is to estimate comparatively the total antioxidant ability of medicinal plants collected in the region of Malesevo Mountain, by two different methods. Total antioxidant capacity (TAC) of different herb infusions was evaluated using the FRAP method (Ferric reducing/antioxidant power) and new electrochemical method by means of cyclic voltammetry. Tea infusion samples were prepared from: Origanum vulgare L. (mountain tea), Mellisa officinalis L. (lemon balm), Hypericum perforatum L. (St. John’s wort), Thymus serpyllum L. (Wild thyme) and Mentha x piperita L. (Mint tea). The results from FRAP method showed that mountain tea has the highest total antioxidant capacity of 27.45, expressed as mmol Fe2+ per liter. Next in the line are: lemon balm with TAC value of 19.54, St. John’s wort tea 12.64, wild thyme 9.45 and mint tea 8.14. Electrochemical method is based on measuring the rate of the homogeneous redox rection of the antioxidants with electrochemically generated ABTS•+ radical (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) in an ethanol/water electrolyte solution at glassy carbon electrode by means of cyclic voltammetry. The electrochemical results are in accord with the data obtained with the FRAP method. Both methods clearly reveal that medicinal plants originating from our country exhibited the strongest antioxidant potential, justifying their usage as potent natural antioxidant agents.

Item Type: Conference or Workshop Item (Poster)
Uncontrolled Keywords: antioxidant capacity, plants, FRAP, cyclic voltammetry
Subjects: Medical and Health Sciences > Other medical sciences
Divisions: Faculty of Medical Science
Depositing User: Viktorija Maksimova
Date Deposited: 18 Feb 2013 10:33
Last Modified: 18 Feb 2013 10:33
URI: http://eprints.ugd.edu.mk/id/eprint/5281

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