Application of MALDI-TOF-MS for detection of pigments in wine

Ivanova, Violeta and Dörnyei, Ágnes and Márk, László and Kilár, Ferenc and Stafilov, Trajče and Vojnoski, Borimir and Stefova, Marina (2010) Application of MALDI-TOF-MS for detection of pigments in wine. In: 10th International Symposium and Summer School on Bioanalysis, 7-14, July, 2010, Zagreb, Croatia.

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Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) as a new valuable technique with a great impact on wine analysis, has been used for qualitative screening of anthocyanins and derived pigments in wine samples from Vranec, Merlot and Cabernet Sauvignon varieties. MALDI-TOF-MS analysis of the wine samples was performed after solid-phase extraction of the pigments using Zip-Tip C18 stationary phase. Solution of acetonitrile/0.1% trifluoroacetic acid (1:1) was used for preconditioning and elution of pigments, while washing was made with 0.1 % trifuoroacetic acid. 2,4,5-trihydroxyacetophenone (dissolved in acetonitrile/H2O = 1/1) was used as a matrix for wine MALDI screening. MALDI-MS results confirmed the presence of glucoside, acetylglucoside and p-coumaroylglucoside derivatives of delphinidin, cyanidin, petunidin, peonidin and malvidin in the wine samples. Furthermore, pyranoanthocyanins formed by reactions of anthocyanins with pyrivic acid and acetaldehyde, as well as flavan-3-ol-anthocyanin adducts and ethyl-bridged pigments have been detected in the samples. Identification of the pigment peaks was performed on the basis of the targeted fragmentation of the ions of interest (their M+ signals) under positive ion mode.

Item Type: Conference or Workshop Item (Lecture)
Subjects: Natural sciences > Chemical sciences
Divisions: Faculty of Agriculture
Depositing User: Violeta Ivanova Petropulos
Date Deposited: 06 Nov 2012 15:01
Last Modified: 28 Oct 2013 12:34

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