Identification and Quantification of Bis-GMA and Teg-DMA Released from Dental Materials by HPLC

Stefova, Marina and Ivanova, Violeta and Muratovska, Ilijana (2005) Identification and Quantification of Bis-GMA and Teg-DMA Released from Dental Materials by HPLC. Journal of Liquid Chromatography & Related Technologies, 28. pp. 289-295.

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Abstract

Most of the composites and sealants used in dentistry are based on bisphenol A diglycidylether methacrylate (Bis-GMA) and triethylenglycol dimethacrylate (Teg-DMA). Reports revealed that in situ polymerization is not complete and that free monomers can be detected by different analytical methods. We studied residual Bis-GMA and Teg-DMA after in vitro polymerization by high performance liquid chromatography (HPLC) after 24 hr, 7, and 14 days. For quantitative analysis, an RP column (250 � 4.6 mm; 5mm) as stationary phase and isocratic elution with CH3CN:H2O (60 : 40) was used. A calibration curve was constructed using standard solutions of Bis-GMA and Teg-DMA in the concentration range from 1 � 1023 to 5 � 1025 mol/L. The limits of detection (LOD) and quantification (LOQ) for both analytes were determined (for Bis-GMA: 0.0076 and 0.0229 mmol/L; and for Teg- DMA: 0.0123 and 0.0373 mmol/L, respectively). The accuracy of the method was confirmed by standard additions. The HPLC analysis of residual components revealed that Bis-GMA was released from commercial samples in the highest concentration after 14 days. Teg-DMA was detected only in one of all analyzed dental materials.

Item Type: Article
Subjects: Natural sciences > Chemical sciences
Medical and Health Sciences > Other medical sciences
Divisions: Faculty of Agriculture
Depositing User: Violeta Ivanova Petropulos
Date Deposited: 05 Nov 2012 08:21
Last Modified: 28 Oct 2013 12:45
URI: http://eprints.ugd.edu.mk/id/eprint/315

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