Evaluation of oxidative stress and inflammation biomarkers in chronic haemodialysis patients before and after haemodialysis

Ruskovska, Tatjana and Cvetkovska, Jasmina and Antarorov, Risto and Jansen, Eugene (2009) Evaluation of oxidative stress and inflammation biomarkers in chronic haemodialysis patients before and after haemodialysis. In: 17th Meeting of Balkan Clinical Laboratory Federation, 16 - 19 September 2009, Ohrid, Macedonia.

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Abstract

Objectives: Oxidative stress and chronic inflammation are common features in haemodialysis (HD) patients that contribute in their high cardiovascular morbidity and mortality. The aim of this study was to evaluate their values of oxidative stress and inflammation biomarkers before and after HD and to compare them with those obtained for healthy volunteers. Methods: 32 chronic HD patients and 17 healthy nonsmokers were included in the study. Reactive oxygen molecules (ROM) and ferric reducing ability of plasma (FRAP) as biomarkers for oxidative stress were determined by photometric methods. hsCRP was measured by immunoturbidimetric method. Results: Before HD, patients had slightly but not significantly higher concentrations of ROM (347±61 : 317±47 CARR U, p>0,05) and significantly higher values of FRAP (1758±320 : 1390±155 umol/l, p<0,01) than controls. Oxidative index (obtained by subtraction of standardized FRAP variable from standardized ROM variable) was paradoxically low in patients before HD (-2,25±2,56), that could be explained by influence of uremia itself on FRAP assay. After HD, patients had significantly higher concentrations of ROM (385±70, p<0,01) and significantly lower FRAP values (1100±194, p<0,01) than controls, with oxidative index of 3,00±1,90. Along with significant increase of ROM and decrease of FRAP during HD we have also measured a significant increase of hsCRP (p<0,01) for average 20% from starting values, that varied considerably among individuals. Conclusions: Although measurement of oxidative stress and inflammation biomarkers is important for HD patients, a careful evaluation of results is essential, especially in terms of influence of uremia on assays for determination of total antioxidant status.

Item Type: Conference or Workshop Item (Poster)
Subjects: Medical and Health Sciences > Basic medicine
Medical and Health Sciences > Clinical medicine
Medical and Health Sciences > Health sciences
Divisions: Faculty of Medical Science
Depositing User: Tatjana Ruskovska
Date Deposited: 02 Dec 2012 09:35
Last Modified: 02 Dec 2012 09:35
URI: http://eprints.ugd.edu.mk/id/eprint/2726

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