Gao, Xin and Wang, Zhengyuan and Martin, John and Abubucker, Sahar and Zhang, Ziping and Mitreva, Makedonka and Hawdon, John (2010) Identification of hookworm DAF-16/FOXO response elements and direct gene targets. PLoS One, 5 (8). e12289.
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Abstract
Background:The infective stage of the parasitic nematode hookworm is developmentally arrested in the environment and
needs to infect a specific host to complete its life cycle. The canine hookworm (Ancylostoma caninum) is an excellent model for investigating human hookworm infections. The transcription factor of A. caninum, Ac-DAF-16, which has a characteristic fork head or ‘‘winged helix’’ DNA binding domain (DBD), has been implicated in the resumption of hookworm development in the host. However, the precise roles of Ac-DAF-16 in hookworm parasitism and its downstream targets are unknown. In the present study, we combined molecular techniques and bioinformatics to identify a group of Ac-DAF-16 binding sites and target genes.
Methodology/PrincipalFindings:The DNA binding domain of Ac-DAF-16 was used to select genomic fragments by in vitro
genomic selection. Twenty four bound genomic fragments were analyzed for the presence of the DAF-16 family binding
element (DBE) and possible alternative Ac-DAF-16 bind motifs. The 22 genes linked to these genomic fragments were
identified using bioinformatics tools and defined as candidate direct gene targets of Ac-DAF-16. Their developmental stage- specific expression patterns were examined. Also, a new putative DAF-16 binding element was identified.
Conclusions/Significance: Our results show that Ac-DAF-16 is involved in diverse biological processes throughout
hookworm development. Further investigation of these target genes will provide insights into the molecular basis by which Ac-DAF-16 regulates its downstream gene network in hookworm infection.
| Item Type: | Article |
|---|---|
| Subjects: | Medical and Health Sciences > Basic medicine |
| Divisions: | Faculty of Medical Science |
| Depositing User: | Mirjana Kocaleva Vitanova |
| Date Deposited: | 30 Nov 2012 15:23 |
| Last Modified: | 30 Nov 2012 15:23 |
| URI: | https://eprints.ugd.edu.mk/id/eprint/2679 |
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