A survey of SL1-spliced transcripts from the root-lesion nematode Pratylenchus penetrans

Mitreva, Makedonka and Elling, Axel A. and Dante, Mike and Kloek, Andrew P and Kalyanaraman, A. and Aluru, S. and Clifton, Sandra W. and Bird, David M. and Baum, Thomas J. and McCarter, James P. (2004) A survey of SL1-spliced transcripts from the root-lesion nematode Pratylenchus penetrans. Mol Gen Genomics, 272 (2). pp. 138-148.

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Plant-parasitic nematodes are important and cosmopolitan pathogens of crops. Here, we describe the generation and analysis of 1928 expressed sequence tags (ESTs) of a splice-leader 1 (SL1) library from mixed life stages of the root-lesion nematode Pratylenchus pene- trans. The ESTs were grouped into 420 clusters and classified by function using the Gene Ontology (GO) hierarchy and the Kyoto KEGG database. Approxi- mately 80% of all translated clusters show homology to Caenorhabditis elegans proteins, and 37% of the C. ele- gans gene homologs had confirmed phenotypes as as- sessed by RNA interference tests. Use of an SL1-PCR approach, while ensuring the cloning of the 5¢ ends of mRNAs, has demonstrated bias toward short tran- scripts. Putative nematode-specific and Pratylenchus - specific genes were identified, and their implications for nematode control strategies are discussed.

Item Type: Article
Uncontrolled Keywords: Pratylenchus, Expressed sequence tags (ESTs), Comparative genomics, Gene expression, Parasite
Subjects: Medical and Health Sciences > Basic medicine
Divisions: Faculty of Medical Science
Depositing User: Mirjana Kocaleva
Date Deposited: 30 Nov 2012 10:02
Last Modified: 01 Oct 2013 11:47
URI: http://eprints.ugd.edu.mk/id/eprint/2567

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