Biological Properties of Allogenic Articular Chondrocytes on the Surface of Bovine Cartilage Explants in vitro

Kim, Gonhyung and Okumura, Masahiro and Bosnakovski, Darko and Ishiguro, Taketo and Kadosawa, Tsuyoshi and Fujinaga, Toru (2003) Biological Properties of Allogenic Articular Chondrocytes on the Surface of Bovine Cartilage Explants in vitro. Journal of Veterinary Medicine Series A-physiology Pathology Clinical Medicine, 50. pp. 418-423.

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Abstract

Bovine cartilage explants were co-cultured with or without allogenic chondrocytes for 4 weeks. The attachment of the applied chondrocytes to cartilage after labelling with fluorescence was assessed using a confocal laser microscope. Morphological changes and the production of extracellular matrix (ECM) of co-cultured chondrocytes on intact and damaged surfaces of cartilage were evaluated by histological and immunohistochemical methods. Co-cultured chondrocytes attached to and proliferated on the intact and damaged areas of cartilage, and a new layer was created there. The defects were also filled with ECM produced by the co-cultured chondrocytes. Glycosaminoglycans and collagen type II were detected in the newly formed ECM, and large numbers of rounded chondrocytes were observed at primitive lacunae in this matrix at 4 weeks of culture. The results suggest that chondrocytes have the ability to attach to, to proliferate on and to establish a new matrix on the intact and damaged surfaces of cartilage explants.

Item Type: Article
Subjects: Natural sciences > Biological sciences
Divisions: Faculty of Medical Science
Depositing User: Darko Bosnakovski
Date Deposited: 30 Nov 2012 15:10
Last Modified: 04 Jul 2013 08:50
URI: http://eprints.ugd.edu.mk/id/eprint/2546

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